液相色谱-电喷雾电离/离子阱质谱法分析金线莲中3-吡啶甲醇  

Analysis of 3-pyridinemethanol in Anoectochilus Roxburghii by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry

作　　者：黄可可[1] 黄丽英[1] 吴萍萍[1] 陈圆婷[1] 周伟炜[1] 

机构地区：[1]福建医科大学药学院,福建福州350004

出　　处：《分析测试技术与仪器》2011年第3期129-133,共5页Analysis and Testing Technology and Instruments

基　　金：福建省科技厅重点项目(2009I0009);福建医科大学教授基金项目(01601086)

摘　　要：应用现代提取技术超声-微波协同萃取金线莲中的3-吡啶甲醇,采用固相萃取技术对样品进行前处理,高效液相色谱法-电喷雾电离/离子阱质谱法(HPLC-ESI/MS)对提取物中3-吡啶甲醇进行测定和鉴别.色谱条件:Agilent TC-C18色谱柱(5μm,4.6×250 mm),流动相:甲醇-0.02 mol/L乙酸铵(5∶95,V/V),流速:1 mL/min,检测波长:260 nm.结果表明峰面积与3-吡啶甲醇在浓度1～10μg/mL范围内呈良好线性关系.回收率在92.0%～96.2%之间,相对标准偏差为2.36%.该法简便、准确、快速.3-pyridinemethanol was extracted from Anoectochilus roxburghii by the ultrasonic-microwave assisted extraction（UMAE）.After pretreatment using solid phase extraction（SPE） technology,3-pyridinemethanol was identified and determined by high performance liquid chromatography-electrospray ion traps mass spectrometry.The chromatographic separation was achieved on an Agilent TC-C18 column（5 μm,4.6×250 mm）using methanol-0.02 mol/L ammonium acetate（5∶ 95,V/V）as the mobile phase at a flow rate of 1.0 mL/min,and 260 nm as the detection wavelength.The analyte showed good linear regression relationship between peak area and concentrations in the range of 1～0 μg/mL（r = 0.9996）.The recovery rate was 92.0%～ 96.2% with an RSD of 2.36%.The proposed method is simple,accurate and rapid.

关 键 词：金线莲 固相萃取 液-质联用 3-吡啶甲醇 

分 类 号：O657.63[理学—分析化学]