鲍氏不动杆菌快速检测方法的建立与应用  

Establishment and application of technique for rapid detection of Acinetobacter baumannii

作　　者：王芳[1] 刘军[1] 易滨[1] 雷红[2] 涂显春[1] 孙敏霞[2] 刘琳[1] 赵晓晓[1] 

机构地区：[1]解放军第309医院感染监控科,北京100091 [2]解放军第309医院检验科,北京100091

出　　处：《中华医院感染学杂志》2011年第20期4406-4408,共3页Chinese Journal of Nosocomiology

摘　　要：目的建立鲍氏不动杆菌快速检测方法并应用于临床,为预防控制鲍氏不动杆菌医院感染提供依据。方法以鲍氏不动杆菌recA基因和16S-23SrRNA基因间区序列为目的基因,建立一种快速检测鲍氏不动杆菌的多重聚合酶链反应(PCR)方法。结果该方法对1株鲍氏不动杆菌标准株和12株临床株进行快速检测,结果准确可靠;用该方法直接对临床环境采样标本进行检测,能够检出鲍氏不动杆菌。结论建立鲍氏不动杆菌的快速检测方法,可为控制鲍氏不动杆菌医院感染提供有力工具。OBJECTIVE To establish a multiplex PCR for rapid identification of Acinetobacter baumannii.METHODS A multiplex PCR targeted 16S-23S rRNA intergenic spacer（ITS） gene sequence and recA gene sequence were evaluated.RESULTS A reference strains and 12 clinical isolates of A.baumannii.were identified by this multiplex PCR method.A.baumannii in the environment specimens were also identified by this method.CONCLUSION Rapid detection of A.baumannii offers an effective tool for controlling the outbreak of A.baumannii infection in hospital.

关 键 词：鲍氏不动杆菌 鉴定 基因间区 多重聚合酶链反应方法 

分 类 号：R378[医药卫生—病原生物学]