妇炎康灌肠剂的HPLC指纹图谱  

HPLC Fingerprint Analysis of Fuyankang Enema

作　　者：廖广华 原素 赖小平[2] 谢称石 陈小新[1,2] 

机构地区：[1]广东华南药业集团有限公司,广东东莞523325 [2]广州中医药大学,广州510405

出　　处：《中国实验方剂学杂志》2011年第20期79-82,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:采用高效液相色谱技术建立妇炎康灌肠剂HPLC指纹图谱,同时分析其化学成分的归属。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.04 mol.L-1 KH2 PO4(含0.1%H3 PO4)为流动相进行梯度洗脱,检测波长230 nm,流速1.0 mL.min-1,柱温为30℃。结果:得到分离度较好的妇炎康灌肠剂的指纹图谱,共标定出13个指纹特征峰,方法稳定、重复性好。同时结合复方药物、阴性样品及单味药的物质信息,确定了特征峰的来源。结论:所建立的指纹图谱特征性强、重复性较好,对妇炎康灌肠剂的质量评价有重要参考价值。Objective： To establish fingerprint of Fuyankang enema by HPLC-DAD,and to analyze its chemical component classification.Method： The samples were separated on a reversed phase Kromasil C18 column（4.6 mm×250 mm,5 μm） with gradient elution.The mobile phase consisted of acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate solution containing 0.1% phosphoric acid,the detection wavelength was 230 nm,the flow rate was 1.0 mL·min-1 and column temperature was set at 30 ℃.Result： Thirteen characteristic peaks were obtained in the fingerprint of Fuyankang enema with good separation.The method was stable with great reproducibility.The sources of characteristic peaks in Fuyankang enema were determined with the information of chemicals of compound,negative samples and single medicine.Conclusion： The fingerprint of Fuyankang enema showed a strong characteristic and good reproducibility,and was an important reference for quality evaluation of Fuyankang enema.

关 键 词：妇炎康灌肠剂 指纹图谱 化学特征峰 

分 类 号：R284.1[医药卫生—中药学]