微波协助提取在中药材含量测定中的应用(2)——微波法与药典法测定白芷中欧前胡素含量比较  

Determination of Microwave-assisted Extraction of Chinese Herbs(2) Comparison of Determination of Imperatorin from Angelica dahurica between Microwave-assisted Extraction and Chinese Pharmacopeia Method

作　　者：方婧[1] 付梅红[1] 杨洪军[1] 张贝贝[1] 王祝举[1] 杨岚[1] 张东[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第20期89-91,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划课题(2006BAI09B07-02)

摘　　要：目的:比较微波协助提取法与药典法提取欧前胡素优势。方法:采用Dikma Diamonsil C18色谱柱(4.6 mm×150mm,5μm),流动相为甲醇-水(55∶45),检测波长300 nm,柱温30℃,流速1 mL.min-1。结果:微波提取时间10 min,提取温度80℃,提取溶剂乙醇。欧前胡素在0.01～0.2μg呈良好线性关系(r=0.999 9)。平均回收率97.48%(n=6)。结论:微波提取法较药典法提取欧前胡素更简便快速、结果准确,为一种符合环保绿色理念的含量测定方法,可用于检测白芷药材中有效成分欧前胡素含量。Objective： To establish a microwave-assisted extraction method for the extraction of imperatorin from Angelica dahurica（Fisch.ex Hoffm.）.Comparison of the determination of imperatorin from A.dahurica between microwave-assisted extraction and Chinese pharmacopeia method was carried out.Method： Dikma Diamonsil C18 column（4.6 mm×150 mm,5 μm） was used.The mobile phase consisted of methanol-water（55∶45）.The temperature of column was at 30 ℃.The flow rate was 1.0 mL·min-1 and the detection wavelength was at 300 nm.Result： Microwave extractive time was 10 min,the temperature was at 80 ℃,the extraction solvent was ethanol.The calibration curve was linear in the range from 0.01 to 0.2 μg（r=0.999 9）.The average recovery was 97.48%（n=6）.Conclusion： The method is simple,rapid,as well as precise and reliable,and can be used for the determination of imperatorin from A.dahurica.

关 键 词：微波提取 白芷 欧前胡素 含量测定 

分 类 号：R284.1[医药卫生—中药学]