银黄系列品种高效液相指纹图谱  

HPLC Fingerprint Chromatogram of Yinhuang Series Preparations

作　　者：刘永利[1] 李冬梅[1] 冯丽[1] 袁浩[1] 

机构地区：[1]河北省药品检验所,石家庄050011

出　　处：《中国实验方剂学杂志》2011年第20期102-104,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立银黄系列品种的指纹图谱检测方法。方法:采用C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈与0.1%磷酸溶液进行梯度洗脱,检测波长为318 nm。通过指纹图谱相似度评价软件,对银黄系列品种的指纹图谱进行了相似度计算。结果:银黄胶囊、银黄含片、银黄颗粒、银黄口服液4个剂型指纹图谱整体一致,共有峰数量与比例稍有差别,各剂型10批样品相似度>0.9。结论:所建立指纹图谱检测方法适用于银黄系列制剂的质量控制。Objective： To established the method of Yinhuang series preparations fingerprint chromatogram.Method： In this method,the C18 column（4.6 mm×150 mm,5 μm） was used,and acetonitrile-0.1% phosphoric acid was used as gradient eluent;detection wavelength was at 318 nm.Result： Yinhuang capsules,Yinhuang buccal tablets,Yinhuang granules and Yinhuang oral liquid were consistent with the overall fingerprint chromatogram,but the number and percentage of common peaks slightly different,10 batches similarity of Yinhuang series preparations are greater than 0.9.Conclusion： This method is useful for quality control of Yinhuang series preparations.

关 键 词：银黄系列品种 指纹图谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]