疏肝活血方促进缺血组织HIF-1α,HGF,VEGF mRNA的表达研究  

Promotion of HIF-1α,HGF,VEGF mRNA Expression in Ischemia Limb Muscle by Shugan Huoxue Recipe

作　　者：刘艳玲[1] 郑巧楠[1] 袁明殿[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032

出　　处：《中国实验方剂学杂志》2011年第20期153-156,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30600843)

摘　　要：目的:探讨疏肝活血方对肢体缺血组织中低氧诱导因子-1α(HIF-1α),肝细胞生长因子(HGF),血管内皮生长因子(VEGF)mRNA表达的调节作用。方法:BALB/c裸鼠64只,随机分为疏肝活血方组、模型组和正常组,股动脉结扎法建立裸鼠肢体缺血模型,分别于术后1,2,3,4周观察各组裸鼠肢体缺血状态的改变,应用苏木素-伊红(HE)染色和CD34免疫组化染色观察肌肉组织一般形态学及微血管数的改变,应用反转录多聚酶链反应(RT-PCR)检测HIF-1α,HGF,VEGF基因在缺血肌肉中表达的动态变化。结果:疏肝活血方能明显改善裸鼠肢体的缺血状态;术后2～4周,疏肝活血方组裸鼠缺血肢体肌肉中的微血管数(MVC)明显高于模型组(P<0.01);疏肝活血方组裸鼠缺血肢体肌肉中HIF-1α,HGF和VEGF的mRNA表达明显强于模型组。结论:疏肝活血方具有明显的促血管生成作用,能够明显上调血管新生相关基因HIF-1α,HGF,VEGF的mRNA表达水平。Objective： To investigate the regulation of Shugan Huoxue recipe on hypoxia inducible factor 1α（HIF-1α）,hepatocyte growth factor（HGF）,vascular endothelial growth factor（VEGF） mRNA expression in muscle after limb ischemia.Method： Forty-eight BALB/c nude mice,were randomly divided into Shugan Huoxue recipe group,blank control group and normal group.Nude mice limb ischemia model was established by femoral artery ligation method.Nude mice limb ischemic changes were observed at Week 1st,2nd,3rd and 4th after limb ischemia respectively.Hematoxylin-Eosine（HE） staining and CD34 immunohistochemical staining was used to observe ischemic morphological changes in muscle tissue.Dynamic changes of gene expression of HIF-1α,HGF,VEGF were detected by reverse transcription polymerase chain reaction（RT-PCR） in ischemic muscle.Result： Compared with the blank control group,Shugan Huoxue recipe could improve the state of ischemia in the limb of nude mice.In 2-4 weeks after ischemia,the microvascular count（MVC） in the ischemic limb muscle of nude mice in Shugan Huoxue recipe group was significantly higher than that in blank control group（P0.01）.The mRNA expression of HIF-1α,HGF,VEGF in the ischemic limb muscle of nude mice in Shugan Huoxue recipe group were upregulated compared with those in blank control group.Conclusion： Shugan Huoxue recipe could increase HIF-1α,HGF and VEGF mRNA expression and promote angiogenesis.

关 键 词：疏肝活血方 缺血 MRNA表达 

分 类 号：R285.5[医药卫生—中药学]