寒、热方剂对消炎痛型胃溃疡寒、热证模型大鼠血清GAS,MOT含量的影响  

Influence of Cold or Heat Prescription on GAS,MOT Contents in Serum on Gastric Ulcer Rats Induced by Indomethacin Combined with Cold or Heat Syndrome

作　　者：李冀[1] 柴剑波[1] 李胜志[1] 胡晓阳[1] 肖洪彬[1] 陈宝忠[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国实验方剂学杂志》2011年第20期170-172,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："十一五"国家科技支撑计划项目(2006BAI08B04-03)

摘　　要：目的:探讨消炎痛型胃溃疡寒、热证模型的形成机制及寒、热方剂对该模型的作用及其方证相应的客观规律。方法:Wistar大鼠140只随机分成空白组、病模型组、病证结合模型组、证模型组。采用寒(冰水、氢氧化钠NaOH)、热(8%干辣椒粉的乙醇混悬液)因素,连续ig 7 d,建立胃寒、热证模型。随后给予寒性方剂(大黄黄连泻心汤,含生药1.8 g.kg-1)、热性方剂(理中丸,含生药3.6 g.kg-1)ig,连续4 d。末次给药后禁食30 h,ip消炎痛,建立消炎痛型胃溃疡寒、热证之"病证结合"动物模型。采用放射免疫分析法、酶联免疫法分别检测受试大鼠血清胃泌素(GAS)、胃动素(MOT)的含量,比较寒、热方剂对其含量的影响。结果:各模型组血清GAS含量均显著升高,与空白组比较有显著性差异(P<0.05或P<0.01)。与空白组比较,单消组、寒消组、热消组、单热组MOT含量明显升高(P<0.01),且热因素组升高显著。大黄黄连泻心汤、理中丸可分别显著降低热模型组、寒模型组GAS,MOT含量(P<0.05或P<0.01)。结论:通过"方证相应"理论验证了"病证结合"胃溃疡寒、热证模型的可行性;大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过降低血清GAS,MOT的含量而实现。Objective：To explore the forming mechanism of gastric ulcer model induced by indomethacin combined with cold or heat syndrome.And to observe the basic mechanism of action and objective regularity of ＇formula-syndrome corresponding＇ of cold or heat formula on gastric ulcer model combined with cold or heat syndrome.Method： One hundred and forty Wistar rats were divided into blank group,disease model group,disease combined with syndrome model group,syndrome model group randomly.Adopting cold（ice water,NaOH）or heat（8%dry pepper powder with ethanol liquid）factors,ig 7 d continuously to establish the stomach cold or heat syndrome models.Then ig cold prescription（Dahuang Huanglian Xiexin decoction 1.8 g·kg-1）,or heat prescription（Lizhong pill 3.6 g·kg-1）,was given for 4 d continuously.After the last ig,fasting 30 h,then ip indomethacin was given to establish the gastric ulcer model combined with cold or heat syndrome,so called ＇disease combined with syndrome＇model.Using radiation immune analysis or euzymelinked immunosorbent assay to detect the contents of gastrin（GAS）,motilin（MOT） in serum.Result：Compared to the blank group,the content of GAS in serum was increased remarkably in each model groups（P0.05 or P0.01）.Excepting the cold syndrome group,compared to the blank group,the content of MOT in serum was increased remarkably in each model groups.（P0.01）.And the contents of MOT in serum increase remarkably in the heat factor groups.Dahuang Huanglian Xiexin Decoction or Lizhong Pill could respectively decrease the contents of GAS,MOT in serum of heat-ulcer group or cold-ulcer group obviously（P0.05 or P0.01）.Conclusion：On the basis of the theory of ＇formula-syndrome corresponding＇,duplication of ＂disease-syndrome combination＂ model,i.e.gastric ulcer model combined with cold or heat syndrome,appears to be feasible.The therapeutic action of Dahuang Huanglian Xiexin decoction on heat-gastric ulcer,or Lizhong Pill on cold-gastric ulcer both is likely achieved by de

关 键 词：消炎痛 胃溃疡 寒、热证 胃泌素 胃动素 

分 类 号：R285.5[医药卫生—中药学]