黄连解毒汤中君-使药对黄连-栀子干预2,4-二硝基苯酚热病证候模型的代谢组学  

Effect of 'JUN-SHI' Herbal Compatibility'Coptis chinensis-Gardenia jasminoides' on Fever Syndrome Model Induced by 2,4Dinitrophenol Based on Metabonomics

作　　者：何薇[1] 荆雷[1] 刘树民[1] 刘东敏[1] 黄志桓[1] 祖金祥[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2011年第20期173-178,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展(973)计划(2007CB512608)

摘　　要：目的:基于代谢组学理论,探讨黄连-栀子药对干预2,4-二硝基苯酚致热病证候模型中生物标记物的变化规律。方法:运用UPLC-MS技术手段,利用主成分分析(PCA)和偏最小二乘-判别分析法(PLS-DA)数据解析方法,确定黄连-栀子药对对2,4-二硝基苯酚致热病证候模型大鼠尿液中生物标记物的影响。结果:初步确定2,4-二硝基苯酚致热病证候模型97个生物标志物,其中药对对31个生物标记物具有明显干预作用。结论:应用代谢组学方法,初步说明黄连-栀子药对对热病证候具有治疗作用。Objective：To explore the the effect of ＇Coptis chinensis Gardenia jasminoides＇ on the fever syndrome model induced by 2,4-dinitrophenol based on metabonomics.Method：The metabolites in rats urine of 2,4-dinitrophenol-induced fever syndrome model were determined by UPLC-MS and then analyzed by Principle Component Analysis（PCA） and Partial Least Squares discrimination analysis（PLS-DA）.Result：Ninty-seven biomarkers on the fever syndrome model induced by 2,4-dinitrophenol were found,and the herbal compatibility have effect on 31 biomarkers of them.Conclusion： The result indicated that the effect on fever syndrome model induced by 2,4-dinitrophenol is related to metabonomics.

关 键 词：黄连-栀子 代谢组学 生物标记物 热病证候 2 4-二硝基苯酚 

分 类 号：R285.5[医药卫生—中药学]