醉鱼草苷Ⅳ对大鼠肝星状细胞增殖、活化的影响  

Effects of Buddlejasaponin Ⅳ on Proliferation and Activation of Hepatic Stellate Cells

作　　者：陈月圆[1] 李典鹏[1] 黄永林[1] 卢凤来[1] 刘金磊[1] 文永新[1] 

机构地区：[1]中国科学院广西植物研究所广西植物功能物质研究与利用重点实验室,广西桂林541006

出　　处：《中国实验方剂学杂志》2011年第20期203-206,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西植物研究所科学研究基金(桂植业09009;11011);桂林市科学研究与技术开发计划项目(20110201)

摘　　要：目的:观察醉鱼草苷Ⅳ对大鼠贮脂细胞株(HSC-T6)增殖及活化的影响。方法:用不同质量浓度的醉鱼草苷Ⅳ处理HSC-T6,采用噻唑蓝(MTT)法测定醉鱼草苷Ⅳ对HSC-T6增殖的影响。采用20,14,9.8,6.86 mg.L-1醉鱼草苷Ⅳ干预细胞24 h,逆转录聚合酶链式反应(RT-PCR)法检测Ⅰ型胶原(Col-Ⅰ),Ⅲ型胶原(Col-Ⅲ)mRNA的表达;Annexin V/PI双染色法结合流式细胞术检测醉鱼草苷Ⅳ对HSC-T6凋亡的影响。结果:醉鱼草苷Ⅳ可显著抑制HSC-T6的增殖,且呈明显的量效关系。醉鱼草苷Ⅳ(6.86 mg.L-1)对HSC-T6 Col-ⅢmRNA表达具有显著的抑制作用(P<0.05);醉鱼草苷Ⅳ(9.8 mg.L-1)对细胞分泌羟脯氨酸(Hyp)和HSC-T6 Col-ⅠmRNA表达具有显著的抑制作用(P<0.05,P<0.01)及对HSC-T6具有显著的诱导凋亡作用(P<0.01),从而达到抗肝纤维化的作用。结论:醉鱼草苷Ⅳ可体外抑制HSC-T6的增殖和活化,对肝纤维化有潜在的干预作用,其机制可能与抑制贮脂细胞增殖及细胞外基质的合成有关。Objective： To investigate the effect of buddlejasaponin Ⅳ on proliferation and activation of rat hepatic stellate cell line HSC-T6.Method： HSC-T6 cells was incubated with buddlejasaponin Ⅳ at different concentrations（20,14,9.8,6.86 mg·L-1） for 24 hours.Effect of buddlejasaponin Ⅳ on proliferation of HSC-T6 was observed by MTT assay.Col-I and Col-Ⅲ mRNA expressions were assayed by reverse transcription ploymerase chain reaction（RT-PCR） after incubation.Apoptosis of HSC-T6 was detected by Annexin V/ PI staining combined with flow cytometry method.Result： Buddlejasaponin Ⅳ could inhibit HSC-T6 cell proliferation,in a dose-and time-dependent manner.Buddlejasaponin Ⅳ with 6.86 mg·L-1 inhibited Col-Ⅲ mRNA expression（P0.05）.Buddlejasaponin Ⅳ with 9.8 mg·L-1 decreased Hyp secretion,inhibited Col-I mRNA expression（P0.05） and induced apoptosis significantly（P0.01）,so as to achieve the effect of anti-hepatic fibrosis.Conclusion： Buddlejasaponin Ⅳcould interfere liver fibrosis via inhibiting proliferation and activation of HSC-T6 cells and inhibiting the synthesis of extracellular matrix.

关 键 词：肝纤维化 醉鱼草苷Ⅳ 贮脂细胞 增殖 

分 类 号：R965[医药卫生—药理学]