脂脉宁对动脉粥样硬化家兔氧化应激反应及LDL-R,VCAM-1基因表达的影响  

Effect of Zhimaining on Oxidative Stress and Gene Expression of LDL-R,VCAM-1 in Rabbit with Atherosclerosis

作　　者：刘应柯[1] 张玉峰[2] 刘尚岭[1] 张希卿[1] 

机构地区：[1]中国人民解放军第153中心医院,郑州450042 [2]河南省中医院,郑州450002

出　　处：《中国实验方剂学杂志》2011年第20期214-217,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省自然科学基金项目(061142400)

摘　　要：目的:探讨脂脉宁抗动脉粥样硬化(AS)的作用机制。方法:用高脂饲料复制家兔AS模型,于4周后再灌服脂脉宁4周,检测血脂、氧化指标及主动脉低密度脂蛋白受体(LDL-R)mRNA及血管细胞黏附分子-1(VCAM-1)mRNA的表达。结果:模型组血脂4项及超氧化物歧化酶(SOD)、丙二醛(MDA)均明显较空白组升高,谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)降低,主动脉内膜、斑块明显增厚;脂脉宁0.375,0.188 g.kg-1血脂及MDA含量均明显较模型组降低,GSH,GSH-Px升高,动脉斑块减小,高剂量组效果更明显。模型组LDL-R mRNA表达明显降低,VCAM-1 mRNA表达升高;给药2组LDL-RmRNA表达则明显升高,VCAM-1 mRNA表达降低,高剂量组尤为明显。结论:脂脉宁抗AS作用与其调整脂质代谢、抗氧化及调控LDL-R mRNA及VCAM-1 mRNA表达有关。Objective：To discuss the anti-atherosclerosis mechanism of Zhimaining.Methed： Rabbit atherosclerosis model was reproduced with high fat diet.Zhimaining was fed for 4 weeks when hyperlipidemia had been taken shape in 4 weeks,Index of lipid,oxidation,Low density lipoprotein-R（LDL-R） mRNA and VCAM-1 mRNA were detected.Result： There was a significient increase in lipid,superoside dismatase（SOD） and malandiavdehyde（MDA） and decrease in Gluathine（GSH）,Glutathine peroxidase（GSH-Px） in model group compared with contral group;there was a significient increase in aorta endometrial and plaque in model group compared with contral group.A very significient decrease of lipid and MDA and increase of GSH,GSH-Px in high dose（0.375 g·kg-1） and low dose（0.188 g·kg-1） group were manifested compared with control group.There was a significient increase of the expression of VCAM-1 mRNA and decrease of the expression of LDL-R mRNA both in the Zhimaining groups compared with contral group.Conclution：The anti-atherosclerosis mechanism of Zhimaining may be related with regulating the antioxidant and metabolism of lipid and the expression of LDL-R mRNA and VCAM-1 mRNA.

关 键 词：脂脉宁 动脉粥样硬化 血管细胞黏附分子-1 低密度脂蛋白受体 氧化反应 

分 类 号：R285.5[医药卫生—中药学]