益肝散对D-氨基半乳糖所致小鼠肝损伤的保护作用  

Protective Effect of Yigan Powder on Acute Hepatic Injury Induced by D-Galactosamine in Mice

作　　者：冯承恩[1] 毕宏[2] 李润祥[3] 黄庆芳[1] 梁理[1] 

机构地区：[1]广东药学院中山校区实验中心,广东中山528458 [2]山西省人民医院,太原030012 [3]广州市皮肤病防治所,广州510095

出　　处：《中国实验方剂学杂志》2011年第20期234-236,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究益肝散对D-氨基半乳糖(D-GalN)致小鼠急性肝损伤的保护作用。方法:将72只昆明小鼠随机分成6组,分别是正常对照组、模型组和联苯双酯阳性对照组,益肝散(按生药量计为9.6,19.2,38.4 g.kg-1)3剂量组。给药组小鼠分别ig给药,正常对照组和模型组ig等体积生理盐水,每天1次,连续7 d。末次给药1 h后,除正常对照组外,其余各组小鼠均ip 10%D-氨基半乳糖无菌生理盐水溶液(850 mg.kg-1),24 h后摘眼球采血和取肝、脾组织,计算肝、脾脏指数,检测血清ALT,AST,并观察其肝组织病理变化。结果:益肝散可明显降低急性肝损伤小鼠血清ALT,AST活性和肝脾指数,与模型组比较差异有统计学意义(P<0.05),且能改善肝脏病理损伤,并呈明显的剂量-效应关系。结论:益肝散对氨基半乳糖所致的急性肝损伤模型小鼠有一定的护肝作用。Objective ：To observe the effect of Yigan powder on acute hepatic injury induced by D-galactosamine（D-GalN） in mice.Method： Seventy-two mice were randomly divided into 6 groups（n=12）： normal control group,model control group,positive control group and three dose groups of Yigan powder（38.4,19.2,9.6 g·kg-1）.The mice in treatment groups were administered medicine respectively via gavages,while the others in control and model groups were treated with the same volume of normal saline.After seven days all the mice except those in the normal control group were treated with intraperitoneal injection of 10% D-GalN（850 mg·kg-1）.Twenty-four hours later,the mice were killed and the blood sample and liver、spleen tissue were taken.The index of liver and spleen was calculated.Liver pathological changes were observed and the activities of serum ALT,AST were determined.Result： Acute liver injury could be significantly reduced by Yigan powder.Serum ALT,AST activity,liver and spleen index were significantly lowered compared with those in the model group（P0.05）.Yigan powder could improve the acute liver injury in a dose dependent manner.Conclusion： Yigan powder had protective effect on acute hepatic injury induced by D-galactosamine.

关 键 词：益肝散 D-氨基半乳糖 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]