高效毛细管电泳法测定毛白杨雄花芽中的IAA与GA_3  

Determination of IAA and GA_3 in the Male Floral Buds of Populus tomentosa Carr.by High Performance Capillary Electrophoresis

作　　者：李媛[1] 陈媛梅[1] 郑彩霞[2] 

机构地区：[1]北京林业大学理学院,北京100083 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《分析测试学报》2011年第10期1128-1132,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(30671655)

摘　　要：建立了高效毛细管电泳测定毛白杨雄花芽中生长素(IAA)和赤霉素(GA3)含量的分析方法。以甲醇为溶剂,超声提取毛白杨雄花芽中的IAA和GA3,经分离纯化后用作供试液。研究了缓冲溶液浓度、pH值、分离电压、分离温度及进样时间等因素对分离检测的影响。确定最佳测定条件为:运行缓冲液为pH 8.0的10 mmol/L硼砂和2.5 mmmol/L SDS溶液,50 mbar压力进样5 s,温度为25℃,运行电压22 kV,检测波长200 nm。最佳条件下,IAA和GA3分别在0.04～0.2 mg/L和0.2～1.00 mg/L的范围内呈良好线性,相关系数分别为0.995 2和0.999 8。2种待测物可在10 min内得到完全分离。该方法简单,分离快速,回收率高,可用于同时检测植物样品中的IAA和GA3。A high performance capillary electrophoresis method was established for the determination of indoleacetic acid （IAA） and gibberellic acid-3 （GA3 ） in male floral buds of Populus tomentosa Carr.. The samples were extracted with methanol by ultrasonic. Effects of experimental conditions, such as concentration and pH value of buffer, separation voltage, separation teperature and injection time on the separation efficiency were investigated. The optimal conditions were as follows; runnin8 buffer： pH 8.0 10 mmol/L borate - 2.5 mmol/L SDS, injection time： 5 s with a pressure of 50 mbar, determination temperature： 25 %, applied voltage： 22 kV, detection wavelength： 200 nm. Under the optimum conditions, good linearities between peak areas and concentrations of IAA and GA3 were found in the ranges of 0.04 - 0. 2 mg/L for IAA and 0.2 - 1.00 mg/L for GA3 , with their correlation coefficients of 0. 995 2 and 0. 999 8, respectively. Two target analytes could be completely separated in 10 rain. With the advantages of simple extraction, fast separation and high accuracy, the proposed method could be simultaneously applied in the determination of IAA and GA3 in plant samples.

关 键 词：毛细管电泳 毛白杨雄花芽 IAA GA3 

分 类 号：O657.8[理学—分析化学] Q946.885[理学—化学]