一种具有双重功能的可视化pH荧光探针的合成及光谱研究  

Synthesis and Spectra Properties of a Fluorescent and Colorimetric pH-Indicator

作　　者：徐学涛[1] 杜志云[1] 卢宇靖[1] 黄华容[1] 张瑞瑞[1] 张焜[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006

出　　处：《分析测试学报》2011年第10期1183-1186,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21042003)

摘　　要：以二烯单酮结构为荧光团,酚羟基为脱质子基团,合成了一种具有双重功能的可视化pH荧光分子探针。pH滴定实验表明,探针的紫外吸收和荧光光谱均对溶液的pH值有很强的依赖性,当体系溶液由酸性变为碱性时,探针的紫外吸收光谱发生明显的红移,并伴有溶液颜色的显著变化;荧光光谱强度在酸性条件下随pH值的变化不大,而在碱性条件下随pH值的增大有20倍以上的增加。由Henderson-Hasselbach公式求得探针的解离常数pKa值为8.13,通过对比硫酸奎宁的标准荧光量子产率测得探针的荧光量子产率为0.056。此外,探针还显示出良好的耐光性和抗金属离子干扰能力。A fluorescent and colorimetric pH-indicator 1, 5-bis （1-hydroxy-4-methoxphenyl）-1, 4- pentadiene-3-one was synthesized based on the ketene structure as the fluorogen and phenolichydroxyl as the deprotonation group. The pH titration indicated that the probe displayed strong spectral sensi- tivity to pH in absorption spectra and fluorescence spectra. The absorption spectra were red-shifted and the solution undergoed distinct color changes from yellow to red with pH turning from acidity to basicity. The fluorescence spectra changed little under acidic solution, while there was more than 20-fold increase in the fluorescence emission intensity under basic solution. The fluorescence dissociation constant（ pK, ）was calculated to be 8.13 by using the formula of Henderson -Hasselbach, and the fluorescence quantum yield（in ethanol solution） was found to be 0. 056 by comparing with the fluorescence quantum yield of quinine sulfate in 0.5 mol/L H2SO4. Furthermore, the probe also showed strong light resistant and anti-metal interference capability.

关 键 词：二烯酮 可视化 荧光 pH探针 

分 类 号：O561.3[理学—原子与分子物理]