不同强度脉冲电磁场对去卵巢大鼠股骨骨密度的影响  

Effect of pulsed electromagnetic fields of different intensity on bone mineral density of femur in ovariectomized rats

作　　者：肖登[1] 杨霖[2] 雷中杰[2] 杨永红[2] 高强[2] 何成奇[2] 

机构地区：[1]重庆医科大学附属第一医院康复理疗科,重庆400016 [2]四川大学华西医院康复医学科康复医学四川省重点实验室,成都610041

出　　处：《激光杂志》2011年第5期61-62,共2页Laser Journal

摘　　要：目的:建立去卵巢大鼠骨质疏松模型,通过采用不同治疗强度脉冲电磁场(PEMFs)干预去卵巢大鼠,观察各组大鼠的股骨骨密度的变化,初步确定治疗骨质疏松的最适的治疗强度。方法:按随机的分组原则用密闭信封法将雌性3月龄SD大鼠50只分为5组:SHAM对照组10只、OVX对照组10只、OVX I组10只、OVXⅡ组10只、OVXⅢ组10只。除SHAM对照组以外,对所有动物按文献方法去势造模。OVX I组、OVXⅡ组和OVXⅢ组三组大鼠每天在频率为8Hz,强度分别为0.77mT、3.82mT和9.87mT的磁场环境中照射40min,共30天。SHAM对照组和OVX对照组不干预。各组动物均在满30天后股动脉放血处死,取血清标本送检血清雌二醇水平,右侧股骨作骨密度的测定。结果:OVX对照组大鼠的股骨骨密度均显著低于其它四组大鼠的股骨骨密度(P<0.05或P<0.01)。但SHAM对照组、OVXⅠ组、OVXⅡ组、OVXⅢ组大鼠的股骨骨密度之间的差异无统计学意义(P>0.05)。说明OVXⅠ组、OVXⅡ组、OVXⅢ组三种磁场强度PEMFs均有显著防止去势大鼠股骨骨密度下降的作用,三组间差异无显著性。结论:PEMFs的磁场强度从0.77mT^9.87mT为有效强度,对于去卵巢大鼠的骨密度丢失具有预防性的治疗作用。Objective：The purpose of this study was to observe the effect of different treatment intensity of pulsed electromagnetic fields（ PEMFs） on bone mineral density of femur in ovariectomized rats, so as to find out the treatment intensity for the best therapeutic efficacy. Methods： Fifty female SD rats were randomly divided into five groups： （ 1 ） sham - operated control （ no PEMFs treatment）, （ 2 ） ovariectomized control （ no PEMFs treatment ）,（3 ） ovariectomized I （ PEMFs treatment at 8Hz frequency with 0.77mT intensity, 40min daily for 30days）, （4） ovariectomized II （PEMFs treatment at 8Hz frequency with 3.82mT intensity, 40min daily 30 days）, and （5） ovariectomized Ill （PEMFs treatment at 8Hz frequency with 9.87mT intensity, 40min daily 30 days）. Except for 10 rats of the sham - operated control group, a standardized ovariectomy was used in every rat. Estradiol （ E2） of serum and bone mineral density of femur were assessed at 30 days after PEMFs treatment. Results： The bone mineral density of the OVXo control group were significantly lower than the other 4 groups （P 〈 0.05 or P 〈 0.01 ）, but the difference in bone mineral density was not significant among the other 4 groups （ P 〉 0. 05 ）. that means OVX 1 , OVX II , OVX III groups can prevent bone mineral density of femur . Conclusion： 0. 77mT -9.87mT magnetic intensity can prevent bone mineral density of femur ovariectomized rats effectively.

关 键 词：脉冲电磁场 去卵巢大鼠 骨密度 

分 类 号：R319[医药卫生—基础医学]