兔急性百草枯中毒后血小板内皮细胞黏附分子-1水平对肺损伤的影响  

Pulmonary platelet endothelial cell adhesion molecule-1 （PECAM-1） in rabbits after acute paraquat （PQ） poisoning

作　　者：高渝峰[1] 胡春林[2] 史晶[1] 廖晓星[2] 

机构地区：[1]广州市第十二人民医院急诊科广州市化学中毒救援中心,广东510620 [2]中山大学附属第一医院急诊科

出　　处：《中国危重病急救医学》2011年第10期585-587,共3页Chinese Critical Care Medicine

基　　金：基金项目：广东省医学科研基金项目（A2010501）;广州市医药卫生科技项目（2009-YB-111）

摘　　要：目的观察急性百草枯（PQ）中毒致肺损伤后血小板内皮细胞黏附分子-1（PECAM-1）水平的变化。方法36只成年新西兰大白兔按随机数字表法分为PQ8、16和32mg／kg3组，每组12只。胃管灌入20oAPQ制模，7d后处死动物取肺组织行苏木素-伊红（HE）染色，观察肺组织病理改变，进行肺损伤评分，Masson染色鉴定肺纤维化程度，免疫组化半定量分析PECAM-1表达；将PECAM-1表达与肺损伤评分、肺纤维化程度进行相关分析。结果随PQ中毒剂量增加，肺损伤评分、肺纤维化程度逐渐加重，肺PECAM-1表达逐渐下降；8、16、32mg／kgPQ中毒组肺损伤评分（分）分别为8．33±1．03、9．83±1．17、11．50±1．38；肺纤维化程度分别为（31．09±2．05）％、（34．37±1．62）％、（36．54±0．44）O；PECAM-1表达分别为（20．31±0．70）％、（19．34±0．68）％、（18．37±0．46）%，组间两两比较差异均有统计学意义（P〈0．05或P〈0．01）。Pearson相关分析显示，PECAM-1表达与肺损伤评分（r=-0．732，P=0．001）、肺纤维化程度（r=-0．779，P〈0．001）均呈明显负相关。结论PQ中毒后，兔肺组织内PECAM-1表达明显下降，呈剂量依赖性，其与肺损伤程度、肺纤维化程度密切相关；PQ对PECAM-1的抑制是肺损伤发生发展的重要因素之一。Objective To examine the changes in PECAM-1 expression and its correlation to the level of pulmonary tissue injury in the lungs from rabbits exposed to acute PQ poisoning. Methods Three groups of New Zealand rabbits （12 each, randomly assigned） were treated with PQ at 8, 16 and 32 mg/kg respectively through gavage. The animals were sacrificed 7 days after the poisoning and the upper lobe of their lungs collected for semi-quantitative microscopic evaluation of tissue injury, pulmonary fibrosis and the expression of PECAM-1 after hematoxylin-eosin （HE）, Masson, and immuno-histological staining. The correlation analysis was used for the relationship between the expression of PECAM-1 and lung injury score or fibrosis of lung. Results The evaluation scores （demonstrated as mean＋ standard deviation） in the three treatment groups were found to be （a） 8.33±1.03, 9.83±1.17 and 11.50±1. 38 for lung tissue injury, （b） （31.09±2.05）%, （34.37±1.62）% and （36.54±0. 44）% for pulmonary fibrosis, and （c） （20. 31± 0.70）%, （19.34±0.68）% and （18.37 ± 0.46）% for PECAM-1 expression. Statistically significant difference （P〈0.05） was found between the results from different dose groups for all the indexes examined. Pearson correlation analysis showed that expression of PECAM-1 was negatively correlated to lung injury score （r=- 0. 732, P=0. 001） and fibrosis degree of lung （r=-0. 779, P〈0. 001）. Conclusion （1） The expressions of PECAM-1 in the lungs of PQ treated animals decrease to increased dose of PQ poisoning, （2） such decrease is correlated to the degree of pulmonary tissue injury and fibrosis of lung. It is possible that PECAM-1 expression inhibition be an important factor in the development of lung injury after acute PQ poisoning.

关 键 词：百草枯 中毒 血小板内皮细胞黏附分子-1 肺损伤 

分 类 号：R595.4[医药卫生—内科学]