透明带诱导绵羊精子顶体反应的研究  

The Study of Ovine Sperm Acrosome Reaction induced by Zone Pellucide

作　　者：刘烈琴[1] 顾申[1] 周卫宾[1] 杨飞鸽[1] 张宏[1] 郭勇[1] 刘云海[1] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国农学通报》2011年第23期29-31,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目"MUC1协调GLYCAM1和ISG15调控绵羊子宫容受态机制的研究"(30972228)

摘　　要：为探讨绵羊透明带对其精子顶体反应的诱导作用,试验分为3组:①不经获能处理的精子与透明带共孵育,②精子经获能处理后与透明带共孵育,③不经获能处理的精子与透明带共孵育,然后添加IA23187,来观察透明带对精子顶体反应的诱导效果。结果显示获能与否并不影响透明带对精子顶体反应的诱导效果,添加IA23187后,顶体反应升高,但差异不显著(P>0.05)。说明透明带可诱导绵羊精子(无论获能与否)发生顶体反应,IA23187不能提高透明带对顶体反应的诱导作用。To find out the effects of ovine sperm acrosome reaction induced by zone pellucid,three experiments were designed：first,co-incubation non-capacited sperm with zone pellucid;second,co-incubation capacitation sperm with zone pellucid;third,adding with calcium ionophore A23187 after co-incubation non-capacited sperm with zone pellucid.The results showed that the rates of acrosome reaction induced by zone pellucid are not significant difference whether the spermatozoa were capacited,IA23187 don ＇ t cooperate with zone pellucide to improve the rate of acrosome reaction.

关 键 词：绵羊精子 获能 顶体反应 透明带 钙离子载体 

分 类 号：S814[农业科学—畜牧学]