玉米灰斑病菌毒素对离体玉米叶片电导率的影响  

The Effects of Cercospora zeae-maydis Toxins on Relative Conductivity of Maize Leaves in Vitro

作　　者：王兴云[1] 张新强[1] 张鹏[1] 盛福敬[1] 高历文[1] 柳少燕[1] 王桂清[1] 

机构地区：[1]聊城大学农学院,山东聊城252059

出　　处：《中国农学通报》2011年第24期258-261,共4页Chinese Agricultural Science Bulletin

基　　金：山东省中青年科学家科研奖励基金"玉米与灰斑病菌互作中病斑反应型及超敏反应研究"(2006BS06014);聊城大学大学生科技文化创新基金"玉米灰斑病菌产毒条件的研究"(SRT08090NX2)

摘　　要：为了研究玉米灰斑病菌的产毒条件和致病机理,采用电导率法测定了静止培养下,以Fries培养液为基质,不同培养时间和培养温度下获得的玉米灰斑病菌毒素对离体玉米叶片电导率的影响。结果表明,相同培养温度和培养时间获得的玉米灰斑病菌毒素(浓度25%)处理不同时间对离体玉米叶片电导率的影响,主要随毒素处理时间的延长,电导率提高率表现为下降趋势;不同培养时间、不同培养温度获得的玉米灰斑病菌毒素对离体玉米叶片电导率均有明显的影响,(20±1)℃培养15天获得的玉米灰斑病菌毒素对离体叶片电导率的影响效果最理想。The toxin-generated conditions and the pathogenicity mechanism of Cercospora zeae-maydis have been studied in this article.The mensuration of conductivity method was used to examine the effects of C.zeae-maydis toxins statically cultured in the Fries nutrient fluid under different temperature and different time on the conductivity of maize leaves in vitro.The results showed that as processing time prolongs,the conductivity increasing rates on maize leaves in vitro decreased when treated with the C.zeae-maydis toxins（at the concentration of 25%） cultivated under the same temperature and the same time.Toxins cultivated under different temperature and different time both had a remarkable influence on the conductivity of maize leaves in vitro.This indicated that toxins statically cultured in the Fries nutrient fluid for 15 days under（20±1）℃ have the best effect on the conductivity of maize leaves in vitro.

关 键 词：玉米灰斑病菌 毒素 电导率 离体玉米叶片 培养时间 培养温度 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]