HPLC法同时测定石韦药材中绿原酸、芒果苷和木犀草素的质量浓度  

Simultaneous Determination of Chlorogenic Acid,Mangiferin and Luteolin in Pyrrosia Lingua Leaves

作　　者：李玉华[1] 彭敬东[2] 郑敏[2] 张珍元[2] 

机构地区：[1]毕节学院化学系,贵州毕节551700 [2]西南大学化学化工学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2011年第2期104-107,共4页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：建立了一种反相高效液相色谱法,同时分离石韦药材中绿原酸、芒果苷和木犀草素,并测定其质量浓度.采用超声优化提取石韦的有效成分：Phenomenex Gemini 5μm C18（150×4.6 mm,5μm）色谱柱,甲醇-冰乙酸溶液为流动相,梯度洗脱,流速为0.8 mL/min;并用波长程序进行检测,柱温35℃,外标法定量.结果表明,绿原酸、芒果苷、木犀草素的线性范围分别为0.084-40.00,0.084-40.00,0.084-13.33μg/mL（R〉0.999）,平均回收率为96.3%~97.8%,相对标准偏差小于2.16%.Objective： To separate and determinate chlorogenic acid,mangiferin and luteolin by a reversed-phase high performance liquid chromatography method.Method： Ultrasonic extraction was used to extract the active ingredients of pyrrosia lingua.The column was Phenomenex Gemini 5 μm C18（150×4.6 mm,5 μm）.The mobile phase was a mixture of MeOH-water（adjust to pH 3.0 with CH3COOH）,and the velocity of flow was 0.8 mL/min,gradient elution.A wavelength program was performed,column temperature was 35 ℃.External standard was used.Results： The linearity range was 0.084～40.00,0.084～40.00,0.084～13.33 μg/mL（R0.999） respectively.The recoveries were 96.3%～97.8%,（RSD2.16%）.Conclusion： This method was sensitive,accurate,rapid and reproducible.It could be applied to determine the quality of pyrrosia lingua.

关 键 词：反相高效液相色谱法 石韦 绿原酸 芒果苷 木犀草素 

分 类 号：O657.72[理学—分析化学]