少腹逐瘀汤中7种有效成分的HPLC-PDA分析与评价  

HPLC-PDA analysis of seven effective components in Shaofu Zhuyu Decoction

作　　者：崔文霞[1,2] 束艳[2] 宿树兰[2] 邬璟[1] 段金廒[2] 唐于平[2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]南京中医药大学江苏省方剂研究重点实验室,江苏南京210046

出　　处：《中成药》2011年第9期1538-1542,共5页Chinese Traditional Patent Medicine

基　　金：江苏省高校自然基金重大基础研究资助项目(06KJA36022);江苏省中医药管理局资助项目(LB09016);南京中医药大学青年自然科学基金资助项目(09XZR15);江苏省方剂高技术研究重点实验室建设项目资助(BM2010576)

摘　　要：目的分析评价少腹逐瘀汤(当归、赤芍、川芎、五灵脂、蒲黄、小茴香、干姜、肉桂、延胡索、没药)不同前处理方法对有效成分分析的影响,建立HPLC-PDA法同时测定少腹逐瘀汤中7种成分的定量测定方法。方法 Dikma Diamonsil C18(4.6 mm×250 mm,5μm)柱以乙腈-0.05%乙酸水溶液为流动相进行梯度洗脱,体积流量1.0 mL/min;柱温30℃;检测波长230,3502,70,320 nm。结果测定的7个成分即芍药苷、咖啡酸、阿魏酸、没食子酸、香草酸、异鼠李素-3-O-新橙皮苷、香蒲新苷,在各自质量浓度范围内线性关系良好(r≥0.999 4);平均回收率为92.2%～105.8%,RSD为1.69%～3.65%。结论建立的HPLC分析方法简单可靠,可操作性好,为少腹逐瘀汤的质量控制提供了简便可行的方法。AIM To analyze the effect of different pretreatment methods on the effective components in Shaofu Zhuyu Decoction（Angelicae sinensis Radix,Paeoniae Radix rubra,Chuanxiong Rhizoma,Trogopteri seu Pteromydis Excrementum,Typhae Pollen,Foeniculi Fructus,Zingiberis Rhizoma,Cinnamomi Cortex,Corydalis Rhizoma,Myrrha）,and establish an HPLC-PDA method for simultaneous determination of seven components.METHODS The analysis was carried out on a Dikma Diamonsil C18（4.6 mm×250 mm,5 μm） column with acetonitrile-0.05% acetic acid solution as mobile phase for gradient elution.The flow rate was 1.0 mL/min,the column temperature was maintained at 30 ℃;and the detection wavelength was set at 230,350,270,and 320 nm,respectively.RESULTS The seven compounds in their respective peak areas had good lineatities（r≥0.999 4） and the average recoveries were 92.2%-105.8%,RSD was within 1.69%-3.65%.CONCLUSION This method is simple,reliable,easy to operate,and can be used for the quality control of Shaofu Zhuyu Decoction.

关 键 词：少腹逐瘀汤 有效成分 HPLC—PDA分析 

分 类 号：R927.2[医药卫生—药学]