HPLC法测定不同产地功劳木中3个有效成分  

Determination of three effective components in Mahoniae Caulis from different habitats by HPLC

作　　者：丛悦[1] 刘静[1] 郭敬功[2] 谢欣梅[1] 王艳[1] 李钦[1] 

机构地区：[1]河南大学药物研究所,河南开封475004 [2]河南大学植物逆境生物学重点实验室,河南开封475004

出　　处：《中成药》2011年第9期1560-1562,共3页Chinese Traditional Patent Medicine

基　　金：河南省科技厅2009年重点攻关项目(092102310159);河南大学校内科研基金(2010YBZR004)

摘　　要：目的测定功劳木中3,4,5-三甲氧基苯酚-1-O-β-D-葡萄糖苷(1)e、rythro-syringoylglycerol-8-O-β-D-glucoside(2)、5,5'-二甲氧基落叶松脂醇-4'-O-β-D-葡萄糖苷(3),为功劳木质量控制提供科学依据。方法采用高效液相色谱法,依利特Hypersil-C18柱(4.6 mm×250 mm,5μm);流动相为乙腈-水(14∶86);检测波长280 nm;体积流量1 mL/min;柱温25℃。结果化合物12,,3分别在0.12～2.40μg(r=0.999 5),0.20～4.00μg(r=0.999 6),0.07～1.40μg(r=0.999 6)与峰面积Y线性关系良好,平均加样回收率依次为100.3%,98.8%,97.2%(n=6)。结论该方法快速,简单,重复性好,适用于同时测定功劳木中化合物12,3,。AIM To develop an HPLC method to determine the contents of 3,4,5-trimethoxy phenyl-1-O-β-D-glucoside（1）,erythro-syringoylglycerol-8-O-β-D-glucoside（2）,5,5′-dimethoxylariciresinol-4′-O-β-D-glucoside（3） of Mahoniae Caulis.METHODS The separations were carried out at 25 ℃ on an elite Hypersil-C18 column（4.6 mm×250 mm,5 μm） eluted with acetonetrile-water（14∶ 86）.The flow rate was 1.0 mL/min with detection wavelength 280nm.RESULTS The curves of compound 1,2,3 were linear over the ranges of 0.12-2.40 μg（r=0.999 5）,0.20-4.00 μg（r=0.999 6）,0.07-1.40 μg（r=0.999 6）,respectively.The average recoveries of compound 1,2,3 were 100.3%,98.8% and 97.2%（n=6）,and the RSDs were 0.78%,2.1%and 1.2%,respectively.CONCLUSION The method is rapid,simple,reproducible,and suitable for simultaneous determination of the compound 1,2,3.

关 键 词：功劳木 3 45 -三甲氧基苯酚-1-O-β-D-葡萄糖苷 erythro-syringoylglycerol-8-O-β-D-glucoside 5 5’-二甲氧基落叶松脂醇-4’-O-β-D-葡萄糖苷 HPLC 

分 类 号：R284.1[医药卫生—中药学]