睡眠剥夺应激对大鼠海马的损伤作用及其对探究行为的影响  

Adverse effect of sleep deprivation on hippocampus and exploratory behavior in rats

作　　者：王新兴[1] 梅竹松[1] 刘伟丽[1] 张志清[2] 武磊[2] 弓景波[1] 钱令嘉[1] 

机构地区：[1]军事医学科学院基础医学研究所全军军事认知与心理卫生研究中心,北京100850 [2]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《军事医学》2011年第9期663-665,680,共4页Military Medical Sciences

基　　金：国家科技重大专项(2008ZXJ090-011);国家自然科学基金青年项目(81000584)

摘　　要：目的观察睡眠剥夺应激对大鼠海马的损伤作用,并研究其对大鼠探究行为的影响。方法以改良多平台水环境(MMPM)建立睡眠剥夺模型,设大平台对照组和睡眠剥夺组。分别记录各组大鼠体质量、血浆糖皮质激素(GC)和神经元特异性烯醇化酶(NSE)水平以及乳酸脱氢酶(LDH)活性的变化;常规HE染色法观察海马损伤情况;旷场实验确定大鼠自主探究能力。结果睡眠剥夺5 d和7 d后大鼠血浆GC含量和LDH活性均显著高于对照组。睡眠剥夺3 d后血浆中NSE水平明显高于对照组,并有随时间延长而逐渐升高的趋势。病理染色和旷场实验显示,睡眠剥夺3 d后大鼠即出现海马损伤和探究行为评分降低。结论睡眠剥夺对大鼠海马具有明显的损伤作用,可能是睡眠剥夺大鼠自主探究行为下降的重要原因。Objective To observe the effect of sleep deprivation on hippocampus and exploratory behavior in rats.Methods The modified multiple platform method（MMPM） was used to establish sleep deprivation model.Open-field test was applied to evaluate the behavior of the model rats.Results The sleep deprivation rats showed decreased body massin the 3rd,5th and 7th day compared with the control groups.The glucocorticoid（GC） content and lactate dehydrogenase（LDH） activity in the plasma of the sleep deprivation rats showed a significant reduction from 5th to 7th day compared with the control groups.The neuron specific enolase（NSE） content in the plasma was reduced significantly in sleep deprivation rats compared with the control groups.Hematoxylin and eosin stain of tissue slice showed that sleep deprivation could induce injury to hippocampus in rats.The exploratory behavior of the sleep deprivation rats decreased significantly compared with the control groups.Conclusion Sleep deprivation can suppress the increase in body mass of rats and cause hippocampus injury.With the increase in the sleep deprivation time,the exploratory behavior of the sleep deprivation rats is gradually suppressed.

关 键 词：睡眠剥夺 海马损伤 探究行为 

分 类 号：R338[医药卫生—人体生理学]