4种中药三联用对鳗鲡致病菌的抑制作用  

Inhibitory effect of four kinds of Chinese herbs and triple compounds against main pathogenic bacteria from cultivated eels

作　　者：关瑞章[1,2] 李忠琴[1,2] 郭松林[1,2] 刘宏伟[1,2] 汪黎虹[1] 

机构地区：[1]集美大学水产学院,厦门361021 [2]教育部鳗鱼产业技术工程研究中心,厦门361021

出　　处：《华中农业大学学报》2011年第6期764-767,共4页Journal of Huazhong Agricultural University

基　　金：农业部公益性行业(农业)科研专项经费(nyhyz07-043);福建省科技计划重点项目(2009N0046);福建省教育厅重点项目(JA09157);福建省科技厅子课题(2009N2003-1);厦门市科技局重点项目(3502Z20103023);集美大学创新团队基金(2010A003)

摘　　要：应用超微粉碎法将虎杖、石榴皮、大黄以及五倍子4种中药进行加工处理至粒径为5～10μm后,组成4个三联用的中药复方,针对近年来从福建各地养殖场病鳗中常检测到的9株致病菌,采用琼脂稀释法测定其抑菌最低浓度。试验结果表明:虎杖、石榴皮、大黄和五倍子组合的4个中药复方对9株养殖鳗鲡主要致病菌均有一定的协同抑制作用,其协同效应因子FIC范围在0.38～0.76。其中,联用效果最好的是复方1,所联用的3种中药对9株致病菌均具有显著协同抗菌效应,FIC≤0.5。每个复方的联用3种中药的最低抑菌浓度均比单用浓度降低了79%以上,不但可大大降低用药量,而且用药成本比单用节省47%以上。Rhus chinensis Mill（RCM）,Rheum palmatum Linn（RPL）,Rhizoma polygoni Cuspidati（RPC）,and Punica granatum Linn（PGL）,were first ground into 5-10 μm superfine powder with ultra-pulverization.Then they were mixed one by one based on chessboard experimental method to get four kinds of compounds of Chinese herbs.To study inhibitation activity of the compounds,the minimum inhibitory concentration（MIC） and the minimum bactericidal concentration（MBC） of single drug and the triple compounds against nine strains of pathogenic bacteria from eels were measured with agar dilution method.The results showed that four kinds of Chinese herbs had certain antibacterial effects on nine bacteria.RCM had the best antibacterial effect,followed by PGL and RPL,and RPC had little antibacterial effect.Four triple combinations had synergism antibacterial effects on pathogenic bacteria,and the value of FIC was from 0.38 to 0.76.Antibacterial synergistic effect of prescription No.1 was the best,whose value of FIC is less than or equal to 0.5 against nine strains of pathogenic bacteria.The inhibitory concentration of each triple compounds reduced at least 79%,and the cost calculated in aquaculture production was lower than single Chinese herbs at least 45%.

关 键 词：鳗鲡 致病菌 中药 抑菌协同效应 

分 类 号：S943[农业科学—水产养殖]