Isolation of Mimosine Degrading Bacteria from Rumen Juice and Mass Production by Gottingen Bioreactor Technology  

作　　者：A. Aung U. ter Meulen F. Gessler H. Bohnel 

机构地区：[1]Institute of Applied Biotechnology in the Tropics, Georg-August-University, Gottingen 37079, Germany [2]Department of Physiology, and Biochemistry, University of Veterinary Science, Yezin, Myanmar [3]Institute of AnimaI Breeding and Genetics, Georg-August-University, Gottingen 37075, Germany

出　　处：《Journal of Agricultural Science and Technology(A)》2011年第5期764-772,共9页农业科学与技术（A）

摘　　要：A method is described to isolate mfinosine degrading bacteria from mmen juice from German steers which had never been ted on either leucaena or mimosine. The isolate was cultivated in continuous culture in a bench top fermenter in Medium 98-5 amended with an increasing amount of mimosine for 16 days. Degradation of mimosine was determined with a colorrinetfic method Incubated at 39 ℃ under anaerobic conditions, the isolate showed complete degradation of mimosine within one ~veek. The bacteriological pure isolate is an aero-tolerant gram-negative cocco-bacillus. By DNA sequencing analysis it belongs to genus Klebsiella. It was multiplied hi brain-heart-infusion, entrapped in alginate, and dried （37 ℃）. Feeding trials in sheep in Myalmlar showed complete innocuity＇ and complete detoxification of mimosine from leucaena leaves.

关 键 词：LEUCAENA MIMOSINE KLEBSIELLA runninants toxicity. 

分 类 号：S823.5[农业科学—畜牧学] Q523[农业科学—畜牧兽医]