利用多重PCR技术鉴定小麦背景中的1BL·1RS易位和Glu-D1d基因(英文)  

Multiplex PCR Identification of 1BL·1RS Translocation and High Molecular Weight Glutenin Allele Glu-D1d in Wheat

作　　者：刘东涛 陈荣振 冯国华 刘世来 王来花 张会云 李德民 王静 

机构地区：[1]徐州农业科学院,江苏徐州221121

出　　处：《麦类作物学报》2011年第4期582-589,共8页Journal of Triticeae Crops

基　　金：Science and technology support program of Jiangsu province(BE2009302-2);Agricultural science and technology innovation fund of Jiangsu province(CX(09)635)

摘　　要：高分子量谷蛋白亚基(HMW-GS)对小麦面粉加工品质有促进作用,尤其是Glu-D1d基因编码的1Dx5+1Dy10亚基能增加面团的筋度和弹性。小麦背景中的1BL.1RS易位对小麦面粉加工品质有显著的负面影响。因此,在小麦品质育种中如何判定小麦背景中是否含有1BL.1RS易位和HMW-GS的Glu-D1d基因具有重要意义。本研究利用3对分别检测1BL.1RS易位、Glu-B3和Glu-D1位点的共显性特异标记,结合SDS-PAGE鉴定,对16份已知遗传背景和Glu-D1x等位基因材料及38株(周麦18×烟农19)F2群体进行了分析,探索出适合同时鉴定小麦背景中1BL.1RS易位和Glu-D1d基因的多重PCR技术实验体系,并采用该体系对国内外352份小麦品种(系)进行了鉴定。结果表明,该体系是同时鉴定小麦背景中1BL.1RS易位和Glu-D1d基因的一种非常有效、简便可行的实验方法,可在标记辅助选择(MAS)育种中应用。Wheat bread-making quality is greatly affected by high molecular weight glutenin subunits（HMW-GS） and 1BL·1RS translocation.Of the glutenins,subunits 1Dx5＋1Dy10 encoded by the Glu-D1d gene have the largest positive effect on dough strength.And serious defects in bread quality have been associated with the presence of 1BL·1RS translocation.Therefore,it has been important to incorporate the Glu-D1d gene into bread wheat and simultaneously avoid the 1BL·1RS translocation.In this paper,a multiplex-PCR,composed of two sets of co-dominant markers,was developed to identify the Glu-D1d gene and the 1BL·1RS translocation.With the advantage that the multiplex PCR could simultaneously distinguish homozygous genotype from heterozygous at both loci,it＇s very useful and efficient in molecular assistant selection（MAS） breeding.It was successfully applied to scan a small size segregating F2 population,and the results were consistent with that of the protein electrophoresis（SDS-PAGE）.Furthermore,the multiplex PCR was validated in a collection from all the ten wheat zones in China and abroad by comparing simultaneously with the PCR assays using the markers of single locus.

关 键 词：小麦 1BL.1RS易位 Glu-D1d基因 多重PCR 

分 类 号：S512.1[农业科学—作物学] S336