六个小麦品系的抗叶锈性评价  

Evaluation of the Resistance to Leaf Rust of 6 Wheat Lines

作　　者：孙一[1] 胡亚亚[1] 杨文香[1] 刘大群[1] 

机构地区：[1]河北农业大学植物病理学系/河北省农作物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《麦类作物学报》2011年第4期762-768,共7页Journal of Triticeae Crops

基　　金：国家"十一五"支撑计划项目(2006BAD08A05);国家公益性行业(农业)科研专项(200903035)

摘　　要：为探究供试的6个小麦品系对叶锈病的抗性状况及所含的抗叶锈基因,利用苗期基因推导、成株期抗病鉴定和分子标记辅助检测进行综合鉴定。结果表明,6个小麦品系中,cp02-62--8-1-1可能含有Lr3bg、Lr42和Lr44;cp01-27-3-3-177可能含有Lr2c、Lr3bg、Lr16、Lr26和Lr42;cp20-3-3-4-178和cp02-39-2-4-377可能含有Lr16和Lr26;cp20-30-1-5-218可能含有Lr3、Lr3bg、Lr11、Lr17和Lr26;cp20-38-4-3-2具有很好的苗期和成株期抗性,可能含有Lr16、Lr26、Lr37和其他抗叶锈基因。测试的6份小麦材料不含有Lr1、Lr9、Lr10、Lr12、Lr19、Lr20、Lr21、Lr24、Lr28、Lr29、Lr34、Lr35、Lr38、Lr41和Lr47。结果表明,这些小麦品系中含有比较丰富的抗叶锈病基因,具有较好的抗叶锈性。In order to evaluate the leaf rust resistance of 6 wheat lines tested,we did the resistance identification in seedling and adult stages with 42 resistance gene lines as tester,inoculated by 8 path-types of pucciuina triticina.Combined gene postulation with the molecular assisted identification,we could concluded that Lr3ka,Lr42and Lr44 were posttulated in cp02-62——8-1-1;Lr2c,Lr3bg,Lr16,Lr26 and Lr42 were posttulated in cp01-27-3-3-177;Lr16 and Lr26 were present in cp20-3-3-4-178;Lr3,Lr3bg,Lr11,Lr17,Lr26 and Lr42 were present in cp20-30-1-5-218 and cp02-39-2-4-377.cp20-38-4-3-2 may carry Lr16,Lr26,Lr37 and other know or un-known resistance genes to leaf rust.However,the specific bands for resistant genes of Lr1,Lr9,Lr10,Lr12,Lr19,Lr20,Lr21,Lr24,Lr28,Lr29,Lr34,Lr35,Lr38,Lr41 and Lr47 were not be detected with the corresponding primers in the 6 lines.The results indicated that there were quite rich resistant genes to leaf rust in the 6 wheat lines.

关 键 词：小麦 抗叶锈基因 基因推导 分子标记辅助选择 

分 类 号：S512.1[农业科学—作物学] S435.121.43