铁皮石斛挥发油化学成分的SPME-GC-MS分析  

Studies on Essential Oil Composition of Dendrobium officinale by SPME-GC-MS

作　　者：康联伟[1] 宋银[1] 张媛[1] 王喆之[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,西安710062

出　　处：《中华中医药杂志》2011年第10期2279-2281,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技支撑计划项目(No.2006BAI06A15)~~

摘　　要：目的:对药用植物铁皮石斛的挥发油化学成分进行定性定量分析。方法:采用固相微萃取/气相色谱/质谱(SPME-GC-MS)联用技术对铁皮石斛挥发性成分进行分析,利用面积归一化法测定各个成分的相对百分含量。结果:共分离出19个峰,结合保留指数和匹配度最后鉴定了其中的14种化合物,占挥发油总量的78.31%。铁皮石斛挥发油主要含烷烃类、醛类、酮类、烯烃类、醇类和酯类化合物,分别占挥发油总成分的28.57%、28.57%、14.29%、7.14%、7.14%和7.14%。其中的主要成分为反-2-辛烯醛(29.96%)、β-紫罗兰酮(15.78%)、芳樟醇(5.36%)、壬醛(4.39%)、β-环柠檬醛(3.40%)和正癸醛(3.14%)。结论:铁皮石斛挥发油含有多种生物活性物质,值得进一步开发利用。Objective： Qualitative and quantitative analysis of the essential oil composition of Dendrobium officinale.Methods： The essential oil were analysed by SPME-GC-MS,and the contents were calculated with normalization method.Results： Nineteen compounds were separated and fourteen constituents were identified,which represented 78.31% of the oil.The main constituents were alkane,aldehyde,ketone,alkene,alcohol and ester compounds,which constituted about 28.57%,28.57%,14.29%,7.14%,7.14% and 7.14% of the essential oils,respectively.The major components were（E）-2-octenal（29.96%）,β-ionone（15.78%）,linalool（5.36%）,nonanal（4.39%）,β-cyclocitral（3.40%）,and decanal（3.14%）.Conclusion： The essential oil of D.officinale contained many bioactive components and had a wide developing potency.

关 键 词：铁皮石斛 挥发油 固相微萃取/气相色谱/质谱 保留指数 

分 类 号：R284[医药卫生—中药学]