银杏达莫对缺血再灌注家兔心肌凋亡及左心室功能的影响  

Effects of GBE on cardiac myocyte apoptosis and the left ventricular function of rabbits from myocardial ischemia/reperfusion

作　　者：张蓓[1] 刘兴德[1] 

机构地区：[1]贵阳医学院附属医院,贵阳550004

出　　处：《中华中医药杂志》2011年第10期2282-2284,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：贵州省高层次人才科研条件特助经费项目[No.TZJF(2006)12]~~

摘　　要：目的:探讨银杏达莫(GBE)对家兔缺血再灌注(I/R)心肌细胞凋亡、凋亡基因及左心室功能的影响。方法:24只家兔分为对照组、缺血再灌注组(I/R)及银杏达莫组(GBE)各8只,缺血再灌注180min后,TUNEL法观察心肌细胞凋亡,免疫组化观察bcl-2、caspase-3蛋白表达,半定量RT-PCR法检测caspase-3 mRNA、bcl-2 mRNA表达,超声测量家兔左心室Tei及LVEF值。结果:与对照组比较,I/R组和GBE组凋亡细胞灰度值、caspase-3表达、Tei值增高(P<0.05),bcl-2表达、LVEF值降低(P<0.05);与I/R组相比较,GBE组bcl-2表达、LVEF值增高(P<0.01,P<0.05),凋亡心肌灰度值、caspase-3表达及Tei值减少(P<0.01,P<0.05)。结论:GBE可减少I/R心肌凋亡,改善左心室功能,上调抑凋亡基因bcl-2,下调促凋亡基因caspase-3。Objective： To study the effect of Ginkgo biloba extract（GBE） on cardiac myocyte apoptosis,the expression of bcl-2,caspase-3 and the left ventricular function in rabbits with myocardial Ischemia/reperfusion.Methods： 24 rabbits were divided into 3 groups randomly（8 rabbits per group）,namely Sham-operated group（Sham）;ischemia reperfusion group（I/R） and Ginkgo biloba extract group（GBE）.At the moment of 180 minutes after reperfusion,Using TUNEL,immunohistochemical and RT-PCR method for detecting myocardium cell apoptosis,expression of protein bcl-2,and caspase-3 and expression of mRNA bcl-2 and caspase-3;UCG was used and Tei index and ejection fraction（LVEF） of the left ventricular were examined to assess the left ventricular total and systolic function in rabbits.Results： Results cardiac myocyte apoptosis,expression of caspase-3,Tei in rabbits with I/R group and GBE group were higher than those of controls（P〈0.05）.Results： the expression of bcl-2,caspase-3 and LVEF in rabbits with I/R group and GBE group were lower than those of controls（P〈0.05）.Cardiac myocyte apoptosis and the express of caspase-3 in GBE group decreased significantly（P〈0.01,P〈0.05） and the express of bcl-2 in GBE group increased significantly compared with the I/R group.The left ventricular function in GBE improved significantly（P〈0.05） compared with the I/R group.Conclusions： GBE could decrease cardiomyocyte apoptosis significantly and improve left ventricular function significantly.Up-regulating the protein expression of bcl-2 and down-regulating the protein expression of caspase-3 in cardiomyocytes during I/R course may be one of the mechanisms of GBE.

关 键 词：银杏达莫 心肌再灌注 细胞凋亡 左心功能 家兔 

分 类 号：R285.5[医药卫生—中药学]