HMGB1和细胞凋亡在宫颈病变中的表达及意义  

Expression of HMGB1 and Cell Apoptosis in Cervical Lesions and Their Significances

作　　者：孟胜君[1] 龚颖萍[1] 

机构地区：[1]湖南省妇幼保健院妇二科,湖南长沙410008

出　　处：《医学临床研究》2011年第9期1688-1690,共3页Journal of Clinical Research

基　　金：湖南省卫生厅科研课题（B2010-084）

摘　　要：[目的]探讨宫颈病变的高迁移率族蛋白B1（HMGB1）表达和细胞凋亡情况.[方法]分别应用免疫组化和末端核酸转移酶脱氧尿嘧啶标记法（TUNEL）.检测并比较正常宫颈组织、宫颈上皮内瘤变（CIN）各期、宫颈鳞癌ⅠA期中HMGB1的表达和细胞凋亡情况.[结果]HMGB1在正常宫颈、CINⅠ、CINⅡ、CINⅢ、宫颈鳞癌ⅠA期中的阳性率分别为5.0%（1/20）、10.0%（3/30）、30.0%（9/30）、56.7%（17/30）、66.7%（20/30）,正常宫颈与CINⅠ（P=0.52）,CINⅡ与正常宫颈（P=0.03）,CINⅠ与CINⅡ（P=0.05）,CINⅢ与宫颈鳞癌（P=0.43）比较,其表达差异均无统计学意义（P〉0.05）.CINⅡ与CINⅢ比较差异显著（P〈0.05）;TUNEL标记率呈现有意义的进行性减少（P〈0.05）,但CINⅢ与宫颈鳞癌ⅠA期无明显差异（P〉0.05）.[结论]HMGB1和细胞凋亡与宫颈病变的转归有关,可能对宫颈病变的预后判定有意义.[Objective] To explore the expression of high mobility group protein B1 （HMGB1） and ceil apoptosis in cervical lesions. [Methods] Immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling（TUNEL） were used to detect the expression of HMGB1 in normal cervix, variant stages of cervical intraepithelial neoplasia（CIN） and IA stage of cervical squamous cell carcinoma and cell apoptosis. [Results]The positive expression ratio of HMGB1 in normal cervix, CIN I , CIN Ⅱ, CINⅢ and IA stage of cervical squamous celt carcinoma were 5.0%（1/20）, 10.0%（3/30）, 30.0%（ 9/30）, 56.7%（17/30） and 66.7%（20/30）, respectively. The P between normal cervix and CIN Ⅰ , CIN Ⅱ and normal cervix, CIN Ⅰ and CIN Ⅱ , CIN Ⅱ and CINⅢ, CIN Ⅲ and IA stage of cervical squamous cell carcinoma were 0.52, 0.03, 0.05, 0.04 and 0.43, respectively. TUNEL labeling rate decreased gradually（ P 〈0.05）, but there was no significant difference in the TUNEL labeling rate between CINⅢ and IA stage of cervical squamous cell carci norna（ P 〉0.05）. [Conclusion] HMGB1 and cell apoptosis are correlated with the turnover of cervical lesions and may be used for assessing the prognosis of cervical lesions.

关 键 词：宫颈疾病/病理学 脱噬作用 

分 类 号：R711.74[医药卫生—妇产科学]