嗜水气单胞菌感染后褶纹冠蚌抗氧化[0]因子的变化  

Changes in the levels of the antioxidant factors in Cristaria plicata infected with Aeromonas hydrophila

作　　者：吴丹[1] 李琰[1] 胡宝庆[1] 文春根[1] 

机构地区：[1]南昌大学生物科学系,江西南昌330031

出　　处：《湖南农业大学学报（自然科学版）》2011年第5期531-536,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30960296);江西省科技攻关项目(2004)

摘　　要：用109个细胞/mL的嗜水气单胞菌菌液感染褶纹冠蚌,在注射后3、6、12、24、48 h分别取蚌的血清和肝胰腺,测定其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)的活性和谷胱甘肽(GSH)、过氧化氢(H2O2)、丙二醛(MDA)的含量。结果表明,经嗜水气单胞菌感染后,试验组血清中SOD活性在3 h时与对照组有显著差异;GPX活性分别在12、24、48 h时与对照组差异显著;GSH含量分别在24、48 h时与对照组有显著差异;MDA含量分别在6、12 h极显著高于对照组。试验组肝胰腺中GSH的含量在24 h时显著低于对照组。试验组血清和肝胰腺中H2O2的含量与对照组无显著差异。褶纹冠蚌经嗜水气单胞菌感染后,血清中各抗氧化指标的变化幅度比肝胰腺的大。This paper reported the activities and contents of antioxidant enzymes in blood serum and hepatopancreas from Cristaria plicata infected with Aeromonas hydrophila. For bacterial inoculation, the experimental group was injected with A. hydrophila （109 cell/mL） and the control group was injected with 0.9% NaCl solution. Blood serum and hepatopancrcas were collected after 3, 6, 12, 24 h and 48 h for testing of the activities of superoxide dismutase （SOD） and glutathione peroxidase （GPX） and of the contents of glutathione （GSH）, hydrogen peroxide （H2O2） and malonaldehyde （MDA）. The results showed that there were significant differences in the activities of SOD in blood serum collected 3 h after injecting between experimental and control group; and the activities of GPX in blood serum also showed significant differences between experimental and control group at 12, 24 h and 48 h after injecting. The content of GSH in blood serum from the experimental group were significantly lower than that from the control group both at 24 h and 48 h after injecting, but in the hepatopancreas, GSH contents was significantly lower in the experimental group only at 24 h after injecting. The content of MDA in blood serum of experimental group was significantly higher than that of the control group at 6 h and 12 h after injecting. The content of H2O2 in blood serum and hepatopancreas respectively showed no significant differences between the two groups. In conclusion, infection of C. plicata with A. hydrophila would have more impact on the levels of antioxidant factors in blood serum than those in hepatopancreas.

关 键 词：褶纹冠蚌 嗜水气单胞菌 超氧化物歧化酶 谷胱甘肽过氧化物酶 谷胱甘肽 过氧化氢 丙二醛 

分 类 号：S944.12[农业科学—水产养殖] Q175[农业科学—水产科学]