固相萃取-高效液相色谱法测定甘蔗和土壤中二甲四氯的残留量  

Determination of MCPA residue in sugarcane and soil by solid-phase extraction and high-performance liquid chromatography

作　　者：傅强[1] 杨仁斌[1] 徐浩然[1] 廖海玉[1] 杨周宁[1] 

机构地区：[1]湖南农业大学资源环境学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2011年第5期554-557,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：农业部农药检定所项目(NCR09091)

摘　　要：建立了固相萃取－高效液相色潜法（SPE—HPLC）测定甘蔗和土壤中二甲四氯残留量。样品采用0．04mol／LNaOH提取，中性氧化铝固相萃取柱净化，HPLC—UV检测。二甲四氯在0．05-10mg／L线性关系良好（R2=0．9993）；最低检出浓度为0．01mg／kg，添加水平为0．05、0．1、1．0mg/kg时，甘蔗和土壤中回收率为82．2％～89．1％，变异系数小于10％。在甘蔗植株中，二甲四氯的消解半衰期为1．78～2．69d；土壤样品中，二甲四氯的消解半衰期为2．40～3．64d。An analytical method was developed to extract and determine 2-methyl-4-chlorophenoxyacetic acid（MCPA） residue in sugarcane and soil using solid-phase extraction and high-performance liquid chromatography （SPE-HPLC）. The MCPA residue in sugarcane and soil was dissolved in 0.04 mol/L sodium hydroxide aqueous solution, and purified through basic alumina-SPE column. Then the sample was detected by HPLC-UV. The method could be used to determine the MCPA residue in sugarcane and soil with fine linear relationship （R2=0.999 3） when the concentration range of MCPA was 0.05-10 mg/L and the limit of detection was 0.01 mg/kg. The recovery rates of MCPA from sugarcane and soil fortified with MCPA at 0.05,0.1,1.0 mg/kg were 82.2%-89.1% and 84.0%-85.7% respectively, with relative standard deviation 〈 10%. The results showed that the half-life of degradation for MCPA was 1.78-2.69 d in sugarcane, 2.40-3.64 d in soil.

关 键 词：二甲四氯 甘蔗 土壤 固相萃取 高效液相色谱 

分 类 号：S482.49[农业科学—农药学]