养肝益水颗粒对自发性高血压大鼠肾脏Smad2、Smad3、Smad7表达的影响  

Effects of Yanggan Yishui Granules on Expression of Smad2,Smad3,and Smad7 in Renal Tissues of Spontaneously Hypertensive Rats

作　　者：王波[1] 戴小华[1] 杨帆[1] 董梅[1] 许祖建[1] 邵正斌[1] 顾健霞[1] 汪玲[1] 丁碧云[1] 周宜轩[1] 

机构地区：[1]安徽中医学院第一附属医院心内科,安徽合肥230031

出　　处：《安徽中医学院学报》2011年第5期59-62,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽省自然科学基金项目(090413254)

摘　　要：目的观察养肝益水颗粒对自发性高血压大鼠(spontaneously hypertensive rat,SHR)肾脏smad2、smad3、smad7表达的影响,探讨养肝益水颗粒保护肾脏的机制。方法将40只12周雄性SHR大鼠随机分为模型组、贝拉普利组(每日1.8 mg/kg)、养肝益水颗粒低剂量组(每日5.4 g/kg)、养肝益水颗粒高剂量组(每日27 g/kg),每组10只;并设Wista-rKyoto(WKY)大鼠10只作正常对照组;模型组和正常对照组给予等容积生理盐水灌胃,每天1次,共6周。采用免疫组织化学法检测各组大鼠肾脏smad2、smad3、smad7的表达水平。结果与正常组相比,模型组肾脏Smad2、Smad3的表达明显上调(P<0.01),Smad7的表达明显下调(P<0.01);各治疗组与模型组相比,肾脏Smad2、Smad3的表达明显下调(P<0.01),Smad7的表达明显上调(P<0.01),且养肝益水颗粒低剂量组与贝拉普利组相比疗效相近(P>0.05),养肝益水颗粒高剂量组疗效优于养肝益水颗粒低剂量组与贝拉普利组(P<0.05,或P<0.01)。结论养肝益水颗粒可能是通过下调肾脏Smad2、Smad3和上调肾脏Smad7的表达以阻断转化生长因子及其受体(transforming growth factor-transfor-ming growth factor receptor,TGF-β-TβR)-Smads信号转导通路而抗高血压肾脏纤维化。Objective To observe the effects of Yanggan Yishui Granules（YGYSG） on the expression levels of Smad2,Smad3,and Smad7 in the renal tissues of spontaneously hypertensive rats（SHR）,in an effort to elucidate the mechanism of YGYSG in protecting the kidney.Methods Fifty 12-week-old male SHRs were averagely randomized into model group,Benazepril（1.8 mg/kg daily） treated group,low（5.4 g/kg daily）-,and high（27 g/kg daily）-dose YGYSG treated groups;and another ten Wistar-Kyoto（WKY） rats were used as normal controls.The rats in model group and control group were treated with normal saline,once daily.Six weeks later,the expression levels of smad2,smad3,and smad7 in the renal tissues were assayed using immunohistochemistry.Results Compared with those of the control group,the expression levels of Smad2,and Smad3 in the renal tissues were significantly increased in the model group（P0.01）,while the expression levels of Smad7 were significantly reduced（P0.01）.Compared with those of the model group,the expression levels of Smad2,and Smad3 in the renal tissues were significantly reduced（P0.01）,while the expression levels of Smad7 were significantly increased in the three treated groups（P0.01）.There was no significant difference in the expression levels of Smad2,Smad3,and Smad7 between low-dose YGYSG treated group and Benazepril treated group（P0.05）;while the difference was significant between high-dose YGYSG treated group and low-dose YGYSG treated group（P0.01）,and between high-dose YGYSG treated group and Benazepril treated group（P0.05）.Conclusion YGYSG can protect the kidney from fibrosis by blocking transforming growth factor-transforming growth factor receptor-Smads signaling pathway via down regulating the expression levels of Smad2,and Smad3,and up regulating the expression levels of Smad7 in the renal tissues.

关 键 词：养肝益水颗粒 自发性高血压大鼠 SMAD2 SMAD3 SMAD7 

分 类 号：R544.1[医药卫生—心血管疾病] R285.5[医药卫生—内科学]