大孔型疏水整体柱的制备及其表征  

PREPARATION AND CHARACTERIZATION OF MACROPOROUS HYDROPHOBIC INTERACTION MONOLITHIC COLUMN

作　　者：王满意[1,2] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]中粮集团科学研究院粮油研发中心,北京100020

出　　处：《离子交换与吸附》2011年第5期410-415,共6页Ion Exchange and Adsorption

基　　金：国家自然科学基金20636010;20876011;20806006;21076017;北京市科委Z09010300840901;863项目2007AA100404;2007AA10Z360;2010AA101506;973项目2007CB714305

摘　　要：利用液体致孔剂正庚烷,将双甲基丙烯酸乙二醇酯(EDMA)和甲基丙烯酸缩水甘油酯(GMA)聚合成含有环氧基团的大孔型整体柱,用正丁胺修饰制备成疏水性整体柱。压汞法分析表明,疏水整体柱的孔隙率为60.2%,500nm以上的大孔占疏水整体柱孔隙率的65.7%。在2890cm/h的高流速下,疏水整体柱的背压只有10.9MPa。以牛血清白蛋白(BSA)为研究对象,动态吸附容量为14.4mg/g介质,在一定范围内,分离效果不受操作流速的影响。此外,在1445cm/h的流速下,3min内即可对细胞色素C、RNase A、溶菌酶和鸡卵清蛋白进行基线分离。结果表明,大孔型疏水整体柱可用于蛋白质的快速分离和分析。Ethylene dimethacrylate and glycidyl methacrylate were employed to prepare monolithic column followed by modification with n-butylamine to afford macroporous hydrophobic interaction monolith, in which n-heptanes was used as porogen solvent. Mercury intrusion analysis showed that 65.7% pores of the monolith had the diameter larger than 500rim, which contributed the lower pressure drops of 10.9MPa even at the velocity of 2890cm/h. The recovery of BSA on the hydrophobic interaction monolith was higher than 90% and dynamic binding capacity was 14.4rag BSA/g media which almost kept as the constant with various operating velocities of mobile phase. Base-line separation of cytochrome C, Rnase A, lysozyme and chicken egg white albumin was achieved within 3rain at flow rate of 1445cm/h and the result was the same as that operated at flow rate of 361cm/h. These indicated the hydrophobic interaction monolith column was promising for fast separation and purification of proteins.

关 键 词：整体柱 疏水作用 快速分离 BSA 

分 类 号：O658[理学—分析化学]