醋柳黄酮对高血压大鼠主动脉环舒缩反应的影响及机制  

Vasodilating effects of total flavones of hippophae rhamnoides on aorta rings in hypertensive rats

作　　者：高天林[1] 余琴[1] 刘应才[1] 蒋毅[1] 谢雪梅[1] 

机构地区：[1]泸州医学院附属医院心内科,四川泸州646000

出　　处：《重庆医学》2011年第29期2955-2958,共4页Chongqing medicine

摘　　要：目的探讨腹主动脉缩窄高血压大鼠服用醋柳黄酮(TFH)治疗后胸主动脉环的舒缩反应及机制。方法 采用腹主动脉缩窄法制备高血压大鼠模型,随机分为4组,每组10只:模型组饮用水10 mL/kg;TFH组TFH 30 mg/kg;卡托普利组(CP组)CP 100 mg/kg;TFH+CP组TFH 20 mg/kg和CP 60 mg/kg,连续灌胃给药,每日1次,10只假手术组作为对照组。用药6周后,进行主动脉环舒缩反应测定,检测血一氧化氮(NO)浓度。结果 TFH、CP能明显升高高血压大鼠的血NO水平(P<0.05),以TFH更明显,且TFH和CP有协同作用。用药组大鼠内皮依赖性舒张敏感性较模型组显著增加(P<0.05),用药组之间差异无统计学意义。NO合成酶抑制剂左旋硝基精氨酸甲酯(L-NAME)预处理后,对乙酰胆碱(Ach)诱发的舒张反应,TFH+CP组明显高于模型组(P<0.05)。对重酒石酸去甲肾上腺素(NE)诱发的收缩反应,TFH+CP组显著低于模型组(P<0.05)。TFH组和CP组在NE浓度较低时收缩反应低于模型组(P<0.05)。结论 TFH长期服用可以改善高血压大鼠内皮舒张功能,降低血管对缩血管物质的敏感性,其机制与保护血管内皮和刺激内皮源性NO增加有关;TFH的舒血管作用与卡托普利等效,两药联用具有协同作用。Objective To explore the mechanism of total flavones of hippophae rhamnoides（TFH）in treating abdominal aortic coarctation-induced hypertension in rats and detect the contractile relaxant response of aorta rings.Methods Experimental hypertensive rat models were established by partially banding abdominal aortic artery and were randomly divided into hypertensive model group（n=10,potable water 10 mL/kg）,TFH group（n=10,30 mg/kg）,Captoprill（CP）group（n=10,100 mg/kg） and TFH＋CP group（n=10,TFH 20 mg/kg＋ Captoprill 60 mg/kg）for 6 weeks while sham-operating as control group（n=10,potable water 10 mL/kg）.Then,detect the contractile relaxant response of aortic rings and serum Nitric Oxide（NO）levels.Results Compared with model group,both TFH group and captopril group obvioureasly increased the level of serum NO（P0.05）,especially TFH group.And there was synergetic effect of TFH and Captoprill.The vasodilation effects of 3 treated groups increased in varying degree compared with the model group（P0.05） while there were no significant differences among those groups.Pretreated with L-NAME（an inhibitor of NOS）,the relaxant response induced by Acetylcholine（Ach） of TFH＋CP group was significantly higher than that of the model group（P0.05）.The contractile response of induced by noradrenalini bitartas（NE） of TFH＋CP group was obviously lower than that of the model group（P0.05）.In low concentration of NE,the contractile response of TFH and CP groups decreased in varying degree compared with the model group（P0.05）.Conclusion TFH long-term use can improve the endothelial function of hypertensive rats,reduce vascular sensitivity to the vasoconstrictor substance.These effects of TFH are mediated by improving endothelial function and increasing the level of serum endothelium-derived NO.The effects of TFH are equal to Captoprill,and there is synergetic effect of them.

关 键 词：高血压 醋柳黄酮 血管舒张 大鼠 

分 类 号：R285.5[医药卫生—中药学]