紫外诱变选育高耐受1,3-丙二醇的克雷伯氏杆菌  

Screening of a Strain Klebsiella pheumoniae with High Resistance of 1,3-propanediol by UV Mutagenesis

作　　者：张宏蕊[1] 王领民[2] 刘树臣[2] 

机构地区：[1]辽宁石油化工大学研究生学院,辽宁抚顺100031 [2]抚顺石油化工研究院,辽宁抚顺100031

出　　处：《广东化工》2011年第10期23-23,40,共2页Guangdong Chemical Industry

基　　金：国家863课题(2006AA020103)

摘　　要：1,3-丙二醇具有许多优良的化学性质,可以作为中间体合成多种有经济价值的工业用化合物,是一种潜在的重要化工平台产品。文章以产1,3-丙二醇的肺炎克雷伯氏菌为出发菌株,通过紫外诱变筛选得到耐受1,3-丙二醇130 g/L的突变株。该突变株以甘油为底物,发酵48 h,发酵液中1,3-丙二醇的质量浓度达到96.57 g/L。1,3-Propanediol has many excellent chemical properties.It was an important potential compound for chemical engineering and a good intermediate to synthesize lots of valuable compounds.A mutant which can resist 1,3-PDO concentration 130 g/L was obtained from its parent Klebsiella pheumoniae by UV.Fermentations with glycerol substrate to produce 1,3-PDO by the mutant were carried out for 48 h,and production amounted to 96.57 g/L.

关 键 词：紫外诱变 1 3-丙二醇 克雷伯氏菌 

分 类 号：TQ658[化学工程—精细化工]