脱落毛发及毛干DNA的STR分型研究  

STR genotyping of tolegen hairs and hair shafts

作　　者：凃政[1,2] 陈松 李万水[1,2] 葛芸英[1,2] 郝金萍[1,2] 赵[1,2] 石屹 

机构地区：[1]公安部物证鉴定中心,北京100038 [2]法医遗传学公安部重点实验室,北京100038

出　　处：《刑事技术》2011年第5期3-7,共5页Forensic Science and Technology

摘　　要：目的探讨脱落毛发及毛干细胞核DNA提取、含量和STR分型问题。方法对脱落毛发或毛干进行DNA提取和定量,使用低扩增体系、增加循环次数和多次平行扩增等方法扩增DNA样本,采用叠加比较的方法分析STR分型。结果 15cm脱落毛发样品DNA含量大于0.3ng的样品占52.8%,STR分型成功率为55.6%;15cm毛干样品DNA含量大于0.3ng的样品占30.6%,STR分型成功率为25%。结论采用增加循环次数、多次平行扩增等LCN-STR分型方法和Mini-STR试剂盒有助于脱落毛发及毛干的STR基因座分型获得。Objective To explore the STR genotyping of tolegen hairs and hair shafts. Methods Nuclear DNA of tolegen hairs or hair shafts was extracted using QIAquick method, quantified using real-time PCR, genotyped using low volume, increasing cycle number and multiple amplifications. Results For tolegen hairs of 15cm, DNA quantity more than 0.3ng was 52.8%, success rate of STR genotyping is 55.6%; for hair shafts of 15cm, DNA quantity more than 0. 3ng was 30.6%, success rate of STR genotyping was 25%. Conclusion LCN-STR and Mini-STR can increase the success rate of STR genotyping from tolegen hairs and hair shafts.

关 键 词：自然脱落毛发 毛干 DNA提取 污染 STR LCN 细胞核DNA 

分 类 号：D919.2[医药卫生—法医学]