羊水栓塞孕鼠血清类胰蛋白酶的变化及意义  

Change and Significance of Serum Tryptase in Pregnant Rats in Amniotic Fluid Embolism

作　　者：侯志刚 宋子亨[2] 赵敏[2] 余舰[2] 税兰[3] 

机构地区：[1]风冈县人民医院,贵州风冈564200 [2]遵义医学院法医学系,贵州遵义563000 [3]遵义医学院附属医院,贵州遵义563000

出　　处：《遵义医学院学报》2011年第4期407-408,412,共3页Journal of Zunyi Medical University

基　　金：红花岗区科技局资助(E-054)

摘　　要：目的在孕鼠注射羊水成份后,观察羊水成份对孕鼠血清胰蛋白酶的动态变化,探讨孕鼠血清胰蛋白酶在羊水栓塞时的意义。方法①取健康妊娠晚期SD大鼠15只,提取自体羊水,离心取上清液;②将孕鼠随机分为3组注入羊水成份(尾静脉注射生理盐水组5只,尾静脉羊水成份注射1小时取血组5只,尾静脉羊水成份注射2小时取血组5只);③右心室内取血,离心分离血清,用ELISA法检测血清中类胰蛋白酶的量。结果类胰蛋白酶表达在羊水成份注入后增加,在2小时组比1小时组更高,与对照组相比,差异均有统计学意义(P<0.05)。结论类胰蛋白酶在羊水成份注入后增加,参与过敏性休克过程。Objective This study was designed to establish fast and convinient mode of pregnant mice injected with amniotic fluid components,so as to observe the dynamic changes of the effect of the composition of the amniotic fluid on the coagulation of pregnant mice.And it can supply clues for the possible mechanisms of the effect of the composition of the amniotic fluid.Methods ①15 SD rats in late pregnancy with gestational age 21 days,were randomly divided into 3 groups： 5 rats for control group（saline group）,5 rats for the group after injected amniotic fluid 1 hour,5 rats for the group after injected amniotic fluid group 2 hour.Amniotic fluid was injected into rat blood circulation respectively through tail vein.The rats were sacrificed in euthanasia after injected 1 and 2 hours respectively.②Indicators test： Got blood from right ventricle.Serum was separated by centrifugal.The secretion of serum tryptase were measured by ELISA.Results ① Compared with the control group,when the amniotic fluid was injected in pregnant mice 1,2 hours,mice lung,kidney,liver,organ coefficient increases,but the difference was not statistically significant.②Tryptase expression increased after the amniotic fluid composition injected.and 2 hours is higher than 1 hours.When compared with control group,difference was statistically significant（P0.05）;③After amniotic fluid composition injected,the pregnant rats PT-INR,PT,TT,APTT prolonged,while the most obvious is APTT.The difference compared with control group was statistically significant（P0.05）.Conclusions Amount raising of tryptase in serum may be a result of intrinsic coagulation pathway activation.

关 键 词：羊水成份 肥大细胞 类胰蛋白酶 

分 类 号：R714.468[医药卫生—妇产科学]