脂肪醇聚氧乙烯醚对细胞的增殖抑制影响  

Proliferation inhibition effects of ethoxylated fatty alcohol on cells

作　　者：陈晓伟[1] 王晓宁[1] 龚 廖青[1] 

机构地区：[1]北京服装学院材料科学与工程学院,北京100029

出　　处：《纺织学报》2011年第10期79-82,共4页Journal of Textile Research

基　　金：北京市属高等学校人才强教计划资助项目(PHR200906122);服装材料研究开发与评价北京市重点实验室项目(2010ZK-07)

摘　　要：针对纺织用化学品安全问题,采用噻唑蓝(MTT)比色法研究了脂肪醇聚氧乙烯醚系列表面活性剂对中华仓鼠卵巢CHO细胞及人体宫颈癌HeLa细胞增殖抑制情况。结果表明:随着环氧乙烷(EO)数量的增加,脂肪醇聚氧乙烯醚对CHO细胞及HeLa细胞增殖抑制率均逐渐减小,脂肪醇聚氧乙烯醚AEO5对2种细胞增殖抑制率最大,AEO9次之,AEO15最小;AEO5浓度大于0.050μL/mL时,细胞增殖抑制率随浓度增大变化不明显,AEO9浓度大于0.075μL/mL时,细胞增殖抑制率随浓度增大变化不明显,而AEO15浓度为0.100μL/mL时,对细胞增殖抑制率仍呈现上升趋势。AEO5、AEO9和AEO15浓度分别低于0.005、0.005、0.010μL/mL时,对CHO细胞及HeLa细胞增殖无明显抑制。In view of the safety problem of chemicals for textiles,MTT assay was adopted to study the effects of three different kinds of ethoxylated fatty alcohols on the proliferation inhibition of Chinese hamster ovary（CHO）cells and human cervical carcinoma HeLa cells.The results indicated that the proliferation inhibition effect of ethoxylated fatty alcohol on both CHO cells and HeLa cells gradually decreased as the number of EO increased.In other words,with respect to the proliferation inhibition on both cells,it showed the following order： AEO5 AEO9 AEO15.When the concentrations of AEO5 and AEO9 were higher than 0.050 μL/mL and 0.075 μL/mL,respectively,the increase of proliferation inhibition effect on the cells exhibited insignificant with the increase of the concentration.However,when the concentration of AEO15 was higher than 0.100 μL/mL,its proliferation inhibition effect on the cells still showed an upward tend.When the concentrations of AEO5,AEO9 and AEO15 were lower than 0.005,0.005 and 0.010 μL/mL,respectively,their proliferation inhibition effect on CHO cells and HeLa cells was not noticeable.

关 键 词：脂肪醇聚氧乙烯醚 CHO细胞 HELA细胞 MTT 增殖抑制 

分 类 号：TQ423.2[化学工程]