基于ZnO/Nafion有机-无机复合膜固定双酶的葡萄糖传感器研究  

Research of ZnO/Nafion-based organic-inorganic composite film fixed two-enzyme glucose biosensor

作　　者：李银峰[1] 欧阳华澍[1] 

机构地区：[1]河南城建学院,河南平顶山467036

出　　处：《河南城建学院学报》2011年第5期36-41,共6页Journal of Henan University of Urban Construction

摘　　要：构建了一种基于ZnO/Nafion复合膜固定双酶的葡萄糖传感器。这种新复合膜的优点是利用ZnO的比表面积大、吸附能力强、具有生物兼容性等性能来固定HRP和GOD酶,同时利用Nafion的成膜、抗干扰能力,制成了响应快速、灵敏度高的葡萄糖传感器。该传感器在最优化实验条件下响应时间小于5 s,以对苯二酚作为电子媒介检测葡萄糖的检测下限为5.0 mmol/L,线性范围为2.5×10-5～3×10-3 mol/L。ZnO Nanorods synthesized by solvothermal route was dispersed in Nafion of sodium hexametaphosphate（（NaPO3）6） to form a stable aqueous dispersion for the fabrication of glucose biosensor.The parameters affecting the fabrication and experimental conditions of biosensors were optimized.The biosensor showed a fast response of less than 5 s and a detection limit of 5.0 mmol/L glucose by using hydroquinone as the mediator.Linear calibration for glucose was obtained in the rage 2.5×10-5 to 3×10-3 mol/L under the optimized conditions.

关 键 词：有机-无机复合膜 葡萄糖 传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]