间歇性气压治疗促进跟腱愈合的实验研究  

Intermittent air pressure therapy to promote healing of achilles tendon

作　　者：陈佳 虞和君[2] 

机构地区：[1]杭州市下沙医院骨科,浙江杭州310018 [2]浙江大学医学院附属邵逸夫医院骨科

出　　处：《全科医学临床与教育》2011年第5期494-496,共3页Clinical Education of General Practice

基　　金：浙江省教育厅课题资助(y200906650)

摘　　要：目的探讨间歇性气压治疗促进跟腱断裂修复的疗效。方法实验分间歇性气压治疗组和未治疗组,建立大鼠跟腱断裂模型后,通过分析比较两组大鼠跟腱愈合横截面积、成纤维细胞计数、糖胺聚糖染色比例及一氧化氮合酶含量等指标评估治疗疗效及跟腱修复机制。结果 3周后跟腱愈合横截面积治疗组为(0.93±0.15)mm2,未治疗组为(0.65±0.06)mm2;光镜下成纤维细胞计数治疗组为(51.23±4.52)个,未治疗组为(30.87±9.87)个;Ⅲ型胶原染色比例治疗组为(12.82±1.89)%,未治疗组为(10.00±0.78)%;糖胺聚糖染色比例治疗组为(89.28±2.35)%,未治疗组为(67.48±5.49)%,一氧化氮合酶蛋白含量治疗组为(217.75±8.78)AU,未治疗组为(170.00±9.54)AU;两组各项结果比较,差异均有统计学意义(t分别=1.58、1.63、1.78、1.81、1.66,P均<0.05)。结论间歇性气压治疗能够有效增加Ⅲ型胶原及糖胺聚糖的表达,增加跟腱愈合横截面积,促进肌腱愈合。Objective To develop a simple physical therapy,intermittent air pressure treatment,to promote tendon healing process.Methods The mice tendon rupture model was generated by dissection the achilles tendon,followed by suture at the same time.After the operation,the mice were treated with or without intermittent air pressure for 3 weeks.The healed tendon cross -sectional area and the number of fibroblasts were measured.The alignment of the collagen fiber was observed under polarized light microscope,with the percentage of different collagen type measured.Results Three weeks＇ treatment with intermittent air pressure therapy enhanced tendon healing,The increased tendon cross-sectional area of treatment group was（0.93±0.15）mm2,while the area of untreated group was （0.65±0.06）mm2.The fibroblast number of treatment group was （51.23±4.52）,and the number of untreated group was （30.87±9.87）.The Type III collagen staining rate of treatment group was （12.82±1.89）%,and the staining rate of untreated group was （10.00±0.78）%.The glycosaminoglycan staining rate of treatment group was （89.28±2.35）%,and the staining rate of untreated group was （67.48±5.49）%.The nitric oxide synthase protein of treatment group was （217.75±8.78）,and the untreated group was （170.00±9.54）.Comparison of all the parameters demonstrated significantly differences between the two groups （t =1.58,1.63,1.78,1.81,1.66,P 0.05）.Conclusions Intermittent air pressure enhanced tendon healing via increasing collagen Ⅲ and glycosaminoglycan expression.

关 键 词：跟腱损伤 气压治疗 物理治疗 

分 类 号：R687.2[医药卫生—骨科学]