SYSMEX XE-5000血细胞分析仪血小板计数性能评价分析  

The performance evaluation of platelet counting in Sysmex XE-5000

作　　者：刘纹[1] 郑炘[1] 刘晓敏[1] 

机构地区：[1]中山大学肿瘤防治中心检验科,广州510060

出　　处：《检验医学与临床》2011年第20期2492-2494,共3页Laboratory Medicine and Clinic

摘　　要：目的评价SYSMEX XE-5000全自动血细胞分析仪对血小板(PLT)检测结果的可靠性。方法取257份乙二胺四乙酸二钾(EDTA-K2)抗凝静脉全血标本,使用鞘流电阻抗法(impedance,PLT-I)进行血小板检测,根据血小板直方图、血小板平均体积(MPV)、红细胞平均体积(MCV)及血涂片法复查,按不同形态分组,分为正常血小板体积组、大血小板组、小红细胞组,分别用荧光法(optical,PLT-O)与显微镜目测法(microscopic,PLT-M)作对比试验。以及另取30份健康体检者的EDTA-K2抗凝静脉全血标本做红细胞碎片干扰试验,运用SPSS16.0及Excel对相关数据进行统计分析。结果正常体积血小板分布组3种方法之间结果无统计学差异;大血小板组PLT-I结果假性降低,PLT-I与PLT-M法结果差异有统计学意义,PLT-O与PLT-M法结果差异无统计学意义;小红细胞组PLT-I结果假性增高,PLT-I与PLT-M法结果差异有统计学意义,PLT-O与PLT-M法结果差异无统计学意义;RBC碎片干扰试验中,PLT-O对红细胞碎片显示出较强的抗干扰能力。结论正常血小板体积标本SYS-MEX XE-5000全自动血细胞分析仪常规使用PLT-I血小板计数结果正确可靠,但血小板直方图、MPV、MCV异常时标本仪器计数血小板不可靠,需与PLT-O或显微镜手工计数法相结合加以纠正。Objective To evaluate the platelet（PLT） counting performance quality of Sysmex XE-5000 automatic hematology analyzer.Methods 257 EDTA-K2 blood samples were counted by XE-5000 with electric impedance（PLT-I）.According to PLT histogram,MPV and MCV,we separated the results to normal platelet,large platelet and microcytes from different PLT shape,and used electric impedance（PLT-I） and microscopic（PLT-M） methods to compare with them.Another blood samples from 60 healthy people were tested with RBC fragment interference experiment.All data were analyzed by SPSS 12.0 and Excel.Results There were significant correlations in the results among the three methods in normal PLT.In large platelet group,PLT-I pseudo reduced.PLT-I and PLT-M method results were statistically significant and PLT-O with the PLT-M method showed no significant difference.Microcytes group increased false in PLT-I.PLT-I and PLT-M method results were statistically significant.The result showed that the PLT-O method had great ability of anti-interference in RBC fragment interference experiment.Conclusion Sysmex XE-5000 automatic hematology analyzer is of high accuracy for normal PLT.PLT-O and PLT-M are better than PLT-I in counting abnormal PLT which PLT histogram,MPV,MCV are unusual.

关 键 词：SYSMEXXE-5000 血细胞分析仪 血小板计数 

分 类 号：R446.113[医药卫生—诊断学]