云南元江干热河谷区植被梯度下土壤真菌群落研究  

Soil Fungal Communities Along the Vegetation Gradient in Hot-dry Valley of Yuanjiang,Yunnan Province

作　　者：易子月[1,2] 石玲玲[1,2] 陈安潜[1] 曹坤芳[1] 邹晓明[1] 

机构地区：[1]中国科学院西双版纳热带植物园热带森林生态学重点实验室,昆明650223 [2]中国科学院研究生院,北京100039

出　　处：《中国农学通报》2011年第22期89-93,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"云南元江干热河谷植被退化机理和重建试验"(90302013)

摘　　要：为了探讨极端环境因素是否会干扰植被对真菌群落的影响,采用克隆文库和变性凝胶梯度电泳(DGGE)的方法对云南元江干热河谷区植被梯度下土壤真菌群落的组成进行了研究。结果表明,尽管植被梯度变化显著,从灌丛过渡到草地,但是地下真菌的群落的变化比较微弱。主要真菌群落由子囊菌门(Ascomycota)组成,占全部克隆的70%。比较显著的群落组成差异是在不同的土层之间。特别是子囊菌门(Ascomycota)和担子菌门(Basidiomycota)这两大类真菌的比例在腐殖质土和矿质土层间呈现很大差异,Ascomycota主要集中在表层腐殖质土中,而Basidiomycota主要分布在矿质土层中。研究结果与其他一般研究发现的植被变化决定土壤微生物变化不同,元江地区极端干燥炎热的环境干扰了地表植被对地面土壤真菌的群落组成,并呈现出与其他地区不一样的群落组成。In order to investigate whether the extreme environmental factors could be important factors in determining the composition of soil fungal communities by interfering with vegetation,we studied the soil fungal communities along the vegetation gradient in Hot-dry Valley of Yuanjiang,Yunnan Province.PCR-DGGE（denaturing gradient gel electrophoresis） and clone library technology were performed in the study.The soil fungal communities showed a very small change from shrub to grass in spite of a significant vegetation gradient existed.Ascomycota was found as the dominant species in the soil fungal communities,accounting for 70% of all clones.There were relatively significant differences in the fungal communities between different soil layers,for example,Ascomycota mainly distributed in the surface humus soil,while Basidiomycota mainly in the mineral soil.Our results demonstrated the soil fungal communities were interfered by the extreme hot and dry environment,showed a quite different community from other general findings,which declared vegetation change decided the soil microorganism.

关 键 词：元江干热河谷 真菌群落 克隆文库 变性凝胶梯度电泳 

分 类 号：S154.36[农业科学—土壤学]