马齿苋离体培养再生体系的建立  

Building-up of Regeneration System in vitro Culture of Portulaca oleracea

作　　者：黄红梅[1] 彭超宇[1] 刘树玲[1] 赵燕[1] 刘清波[1] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128

出　　处：《中国农学通报》2011年第22期136-141,共6页Chinese Agricultural Science Bulletin

基　　金：湖南农业大学大学生创新性实验计划项目"马齿苋组织培养研究"(20060603)

摘　　要：以马齿苋幼嫩叶片和茎段为试验材料,对外植体灭菌、愈伤组织诱导、不定芽分化和试管苗生根的最佳条件进行研究,以期建立马齿苋再生技术体系,并为马齿苋的规模化繁殖和遗传改良等研究奠定基础。结果表明,最佳外植体灭菌方法为:用0.1%的升汞溶液浸泡8min;最佳愈伤组织诱导培养基为MS+2,4-D0.5mg/L+6-BA0.5mg/L;最佳不定芽分化培养基为MS+6-BA5.0mg/L+NAA0.4mg/L;最佳生根培养基为1/2MS+IBA2.0mg/L。To establish the regeneration technique system of Portulaca oleracea which lay the foundation for the research of large-scale propagation and genetic improvement,the optimal conditions for disinfecting of explants,inducing of callus,differentiating of adventitious buds and rooting of test-tube seedlings of Portulaca oleracea were studied by using leaf and stem bearing buds as explants.The results showed that the optimum disinfection method was to put explants in 0.1% HgCl 2 for 8 min;MS＋2,4-D 0.5 mg/L＋6-BA 0.5 mg/L was the best medium to induce callus,MS＋6-BA 5.0 mg/L＋NAA 0.4 mg/L was the best sprout medium.The optimal culture medium was 1/2MS＋IBA 2.0 mg/L for rooting.

关 键 词：马齿苋 组织培养 愈伤组织 

分 类 号：Q943.1[生物学—植物学] S647[农业科学—蔬菜学]