国产决明属生理生化特性与系统分类的关系  

Relationship between Biochemistry Characteristics and System Classification of Cassia in China

作　　者：蔡静[1] 何家庆[1] 汪学敏[1] 舒畅[1] 吴玲[1] 

机构地区：[1]安徽大学生命科学学院/安徽省资源植物研究中心,合肥230039

出　　处：《中国农学通报》2011年第22期142-147,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目(30040027);安徽省环保局专项基金项目(2006-2008);国家大学生创新实验项目(101035714)

摘　　要：以国产决明属(Cassia)4种植物为实验材料,采用愈创木酚法和聚丙烯酰胺垂直板凝胶电泳对其抗逆指标和过氧化物酶(POD)同工酶进行测定,分析其系统树与传统决明属系统分类之间的差异。结果表明:发芽1天的过氧化物酶活性和3天的MDA含量差异显著,可作为探讨其亲缘关系的指标之一。发现POD同工酶带12条,4种决明没有共同拥有的条带,决明属植物的特征性酶带主要分布在b区和a区。与传统分类不同,抗逆指标和同工酶分析结果都表明,槐叶决明(Cassia sophera)与豆茶决明(C.nomame)亲缘关系最近,翅荚决明(C.alata)次之,决明(C.tora)与其他种亲缘关系最远,属于另一支。In order to draw a phylogenetic tree of 4 species of Cassia in China,the study used guaiacol method and polyacrylamide vertical slab gel electrophoresis to test the resistance indicators and peroxidase isozyme,and analyzed the differences between the result and traditional systematics.Peroxidase activity of 1 day,MDA of 3 days significantly differed among species,so it can be used as one of the indicators to explore their genetic relationship.12 POD enzyme bands were found,characteristic enzyme bands were mainly in b-zone and a-zone.Differed from traditional systematics,both resistance indicators and POD zymogram revealed that Cassia sophera was closer to C.nomame than C.alata in systematics,while C.tora belonged to another branch.

关 键 词：决明属 萌发 抗逆生理指标 过氧化物酶同工酶 亲缘关系 生理生化特性 

分 类 号：Q945.79[生物学—植物学]