差示分光光度法测定牛黄类中成药中胆红素的含量  

ASSAY OF BILIRUBIN OF OX GALLSTONE IN CHINESEPATENT MEDICINES BY DIFFERENTIAL SPECTROPHOTOMETRY

作　　者：倪坤仪[1] 屠树滋[1] 俞兵 郑露露 

机构地区：[1]中国药科大学,南京210009 [2]上海中药一厂

出　　处：《药学学报》1990年第10期763-768,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：本文利用胆红素溶液在光照下易氧化变色及其吸收光谱发生显著变化的性质,在波长453 nm处,测其光照前后的吸收度差值(△A),△A与胆红素溶液浓度呈线性关系,对含牛黄的中成药(六应丸,六神丸和牛黄消炎丸)中胆红素进行了定量,并对其它组分进行了光照试验,排除了光照对其它组分的影响,从而确定了此法的可行性。This paper describes that the absorption spectrum of bilirubin solution was changed. obviously by photo-oxidation. The absorbance was measured at 453 nm before and after light irradiation. The contents of bilirubin of ox gallstone in Liuying pills, Liushen pills and Niuhuang Xiaoyan pills were determined by differential absorbance (AA). Calibration graph was linear in the range of 1.6～8.0μg/ml for ΔA. The average recoveries for three Chinese patent medicines were over 93%. Determinations of bilirubin in Chinese patent medicines were not affected by other components.

关 键 词：胆红素 六神丸 牛黄消炎丸 

分 类 号：R286.711[医药卫生—中药学]