白头翁中毛莨甙及合成毛茛甙的高效液相色谱测定法  

DETERMINATION OF RANUNCULIN IN PULSATILLA CHINENSIS AND SYNTHETIC RANUNCULIN BY REVERSED PHASE HPLC

作　　者：张秀琴[1] 刘爱茹[1] 徐礼燊[1] 

机构地区：[1]中国医学科学院药物研究所,北京100050

出　　处：《药学学报》1990年第12期932-935,共4页Acta Pharmaceutica Sinica

摘　　要：白头翁为毛莨科属植物Pulsatilla chinensis,以根入药,其煎剂能抑制阿米巴原虫的生长、杀灭阴道滴虫等作用,用于细菌性痢疾和阿米巴痢疾的治疗。白头翁中主要成分有原白头翁素[protoanemonin,系由毛莨甙(ranunculin)酶解而得]、白头翁素(anemonin)等,其结构如下:A method for the determination of ranunculin in Pulsatilla chinensis and synthetic ranunculin by reversed phase HPLC using betamethasone as internal standard is described. The column employed was a 10μm LiChrosorb RP - 18 (230×4mm), the eluting solvent consisted of methanol-water(40: 10, V/V)and the effluent was monitored at 225nm.The advantages of thismethod are simple, rapid and accurate. The coefficients of variation are less than 1%.

关 键 词：白头翁 毛莨甙 合成 高效液相色谱 

分 类 号：R282.710.3[医药卫生—中药学]