甘肃汉、回族2型糖尿病患者PPARγ2基因Pro12Ala多态性分析  

Analysis of PPAR-γ2 gene Pro12Ala polymorphism between Gansu Han and Hui population with diabetic

作　　者：关晓丽[1] 刘怡[1] 刘一帆[1] 陈慧[1] 

机构地区：[1]兰州大学第二医院,甘肃兰州730030

出　　处：《中国老年学杂志》2011年第19期3669-3671,共3页Chinese Journal of Gerontology

基　　金：甘肃省自然科学基金资助项目(0710RJZA034)

摘　　要：目的分析甘肃汉族和回族2型糖尿病患者PPARγ2基因Pro12Ala多态性(PP、PA、AA)。方法应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)确定PPARγ2基因Pro12Ala多态性的基因型及等位基因频率,比较两民族间的差异。结果两个民族中,Pro12Ala多态性在同民族中2型糖尿病和正常对照组之间差异不具有统计学意义,A等位基因的分布频率在两民族2型糖尿病组中大致相同,分别为4.17%,4.62%(χ2=0.027,P=0.871),2型糖尿病组中基因型为PP者体重指数、空腹血糖、甘油三酯均高于XA(PA/AA)基因型,但差异无统计学意义(P>0.05)。结论在本实验中的甘肃汉族和回族人群中PPARγ2基因Pro12Ala多态性主要以PP型为主,该多态性可能与2型糖尿病发病无关联。Objective To analyze the Pro12Ala polymorphism in PPARγ2 gene between Hui and Han in Gansu province.Methods PCR-RFLP was used to define the distribution of allele and genotype frequencies of Pro12Ala polymorphism in PPARγ2gene.Results There is no statistical difference between type 2 diabetics and control about Pro12Ala polymorphism in the nation.The A allele frequencies of Pro12Ala polymorphism in type 2 diabetes of Hui and Han were 4.17% and 4.26%,respectively（χ2=0.027,P=0.871）.There was no difference in two nationalities,but subjects with PP genotype had higher levels of body mass index,fasting blood glucose and triglyceride than XA（PA/AA） genotype in type 2 diabetes.The difference was not statistically significant（P〉0.05）.Conclusions In this study,Pro12Ala polymorphism in PPARγ2 gene is not significantly associated with type 2 diabetes in Han and Hui population.

关 键 词：回族 汉族 过氧化物酶体增殖物激活受体γ2 基因多态性 

分 类 号：I87[文学]