穴位埋线治疗实验性结肠炎大鼠的分子机制  

Molecular mechanism of acupoint catgut-embedding for experimental colitis in rats

作　　者：张夏毅[1] 沈霖[1] 范恒[1] 廖奕[1] 梁丽[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北武汉430022

出　　处：《中国针灸》2011年第10期913-918,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:30772878;81072944

摘　　要：目的:探讨穴位埋线治疗实验性结肠炎大鼠的作用效应及作用机制。方法:将18只SD大鼠随机分为3组:正常对照组、模型组、穴位埋线组,每组6只。除正常对照组未行造模外,其余两组大鼠均采用三硝基苯磺酸(TNBS)造模。模型组不予干预,正常饮食。穴位埋线组于"上巨虚""天枢""大肠俞"处进行穴位埋线治疗。治疗15d后观察大鼠的腹泻、便血症状和结肠病理组织学改变,用免疫组化染色法观察大鼠结肠组织白细胞介素17(IL-17)、β2肾上腺素受体(β2AR)、核因子κBp65(NF-κBp65)的表达;用Western blot法检测大鼠脾淋巴细胞β2AR和NF-κBp65蛋白的表达。结果:穴位埋线组的大鼠腹泻、黏液脓血便症状得到较快控制,大鼠结肠黏膜组织损伤明显改善。大鼠脾淋巴细胞NF-κBp65的表达,与正常组(86.22±8.09)相比,模型组(249.70±13.66)的表达增多(P<0.01);与模型组相比,穴位埋线组(219.02±7.42)的表达减少(P<0.01)。大鼠脾淋巴细胞β2AR的表达,与正常组(957.45±171.56)相比,模型组(594.97±173.22)的表达减少(P<0.01);与模型组相比,穴位埋线组(1335.93±244.34)的表达增多(P<0.01)。模型组大鼠结肠黏膜组织中IL-17的表达增多,而穴位埋线组中IL-17的表达减少。结论:穴位埋线"上巨虚""天枢""大肠俞"治疗实验性结肠炎有明显效果,其作用机制可能是通过调节IL-17、β2AR、NF-κBp65的表达,发挥其治疗作用。Objective To explore the effect and its mechanism of acupoint catgut-embedding for experimental colitis in rats. Methods Eighteen SD rats were randomly divided into a normal control （NC） group, a model （MO） group and a acupoint catgut-embedding （AC） group, 6 rats in each group. Animals in MO group and AC group were treated by trinitro-benzene-sulfonic acid （TNBS） to establish model with colitis. No other treatment was given to the rats in MO group, but aeupoint catgut-embedding was implanted at ＂Shangjuxu＂ （ST 37）, ＂Tian- shu＂ （ST 25） and ＂Dachangshu＂ （BL 25） in the rats in AC group. The symptoms of diarrhea and bloody stool, and histopathology changes in colon were observed 15 days after the treatment. Expression of IL-!7,I？2AR, NF- κBp65 were observed by immunohistochemistry. Expressions of NF-κBp65 and β2AR in splenic lymphocyte were detected by the Western blot method. Results Diarrhea and mucus bloody purulent stool were soon controlled, and colon mucosa injures were obviously improved in AC group. The NF-κBp65 value in splenic lymphocytes of 249.70±13.66 in MO group was higher than 86.22±8.09 in NC group （P〈0.01）, and 219.02±7.42 in AC group was less than that in MO group （P〈0.01）. The expression of β2AR in splenic lymphoeytes of 594.97± 173.22 in MO group was less than 957.45±171.56 in NC group （P〈0.01）, and 1335.93±244.34 in AC group was higher than that in MO group （P〈0. 01）, The expression of IL-17 in colon mucosa in MO group was increased, while the expression of IL-17 in colon mucosa in AC group was decreased. Conclusion Acupoint catgut- embedding at Shangjuxu （ST 37）, Tianshu （ST 25） and Dachangshu （BL 25） has obviously effect in treating ex- perimental colitis and the mechanism may be related to regulate the expression of IL-17 ,β2AR and NF-κBp65.

关 键 词：结肠炎 穴位埋线 Β2肾上腺素受体 核因子ΚBP65 白细胞介素17 

分 类 号：R245.9[医药卫生—针灸推拿学]