DELLA蛋白参与拟南芥幼苗对一氧化氮逆境的抵抗  

DELLA Contribute to Tolerance to Nitric Oxide Stress in Arabidopsis Seedlings

作　　者：姚涛[1] 白素兰[1] 李苗苗[1] 张耀川[2] 何奕昆[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京农业职业学院,北京102442

出　　处：《植物学报》2011年第5期481-488,共8页Chinese Bulletin of Botany

基　　金：国家重点基础研究发展计划(No.2007CB948201);国家自然科学基金(No.30771094);北京市自然科学基金(No.5082003;No.5112006)

摘　　要：DELLA蛋白是赤霉素信号途径中的一类对植物生长起抑制作用的重要蛋白质,在拟南芥(Arabidopsis thaliana)基因组中已经鉴定出5个DELLA蛋白基因。目前研究发现,DELLA蛋白在抗逆中也起了重要的作用。近年来,一氧化氮(nitric oxide,NO)的研究工作取得重要进展,低浓度的NO能够促进植物的生长,但在高浓度下它对植物生长起抑制作用甚至导致细胞死亡。通过外施一氧化氮供体硝普钠(sodium nitro prusside,SNP),研究高浓度NO对拟南芥幼苗生长的影响,发现植物体内H2O2积累,幼苗死亡。通过研究DELLA蛋白基因表达的变化及其相关突变体的表型,证明DELLA蛋白在抵抗NO逆境中起了重要作用。研究结果揭示了DELLA蛋白与NO逆境的关系,为今后科学指导农业生产提供了理论依据。Five members of DELLAs, important plant growth repressors in the gibberellin pathway, have been described in Arabidopsis. DELLAs play an important role in stress tolerance. A low concentration of nitric oxide （NO） can promote plant growth and development, and a high concentration can inhibit plant growth, even cause cell death. We studied the effect of NO at a high concentration in Arabidopsis seedlings by treatment with the NO donor sodium nitroprusside （SNP）, and the results indicate that the cell death caused by NO is correlated with H2O2 accumulation. Study of phenotypes of serially DELLA-deleted mutants and DELLA gene expression in response to NO revealed that DELLAs contribute greatly to tolerance to NO stress in Arabidopsis seedlings. Thus, we reveal the relationship between DELLA and NO stress in such seedlings, which can contribute to agricultural production.

关 键 词：细胞死亡 DELLA蛋白 一氧化氮 抗逆 

分 类 号：Q945.78[生物学—植物学]