应用关联分析鉴定大豆对斜纹夜蛾的抗性基因  

Identification of Genes with Soybean Resistance to Common Cutworm by Association Analysis

作　　者：王慧[1] 高中杰[1] 张丹[1] 程浩[1] 喻德跃[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,南京210095

出　　处：《植物学报》2011年第5期514-524,共11页Chinese Bulletin of Botany

基　　金：国家重点基础研究发展计划(No.2010CB125906);南京农业大学青年科技创新基金(No.KJ09004)

摘　　要：用135个分布于全基因组的SSR(simple sequence repeat)标记分析196份大豆(Glycine max)地方品种的遗传变异、群体结构和连锁不平衡(linkage disequilibrium,LD)。在考虑群体结构的条件下,应用全基因组关联分析的方法鉴定与大豆抗斜纹夜蛾有关的数量性状位点,进而发掘各关联位点的优异等位基因及代表性载体材料。结果表明:(1)该群体不仅地理起源广,而且遗传变异丰富;(2)在整个群体内,有17.9%的标记对处于显著的连锁不平衡,而且在相同染色体上标记对间连锁不平衡延伸的平均距离约为6.61cM(D'>0,P<0.05);(3)通过关联分析发现,有7个SSR标记分别与3个大豆抗斜纹夜蛾性状关联(P<0.01),其中4个位点对性状的变异解释率超过了10%,6个位点所在的连锁群上存在已报道的食叶性害虫抗性位点;(4)各位点等位基因的效应分析显示,幼虫重相关位点的等位基因主要表现为减效作用,等位基因Sat_334-A208减效作用最大(43.9%);单虫食叶量和蛹重相关位点的等位基因主要表现为增效作用;等位基因Satt199-A186对单虫食叶量增效作用最大(36.4%);等位基因Sat_320-A286对蛹重增效作用最大(31.4%)。We used 135 genome-wide simple sequence repeat（SSR） markers to assess genetic diversity,population structure and linkage disequilibrium（LD） of 196 soybean landraces.On the basis of estimated population structure,we conducted association mapping for soybean resistance to common cutworm（CCW） using genomic-wide mapping strategies and detected the elite alleles of soybean resistance to CCW,along with their carriers.In addition to wide geographic origin,the population showed extensive genetic variation,and 17.9% of the SSR pairs were in LD（with D＇0,P0.05）.The extent of LD was 6.61 cM with genetic distance of locus pairs for the loci in the same linkage group（LG）.Association analysis revealed 7 SSRs associated with soybean resistance to CCW（P0.01）：4 accounted for 10% of the total genetic variation for resistance;6 located in the linkage groups reported to be related to soybean resistance to insects.Allele effect analysis revealed that the alleles related to larval weight of CCW mainly had a negative effect,the allele Sat_334-A208 showing the largest negative effect（43.9%）.The alleles related to leaf consumption of single larva（LCL） and pupal weight（PW） of CCW mainly had a positive effect,the allele Satt199-A186 showing the largest positive effect for LCL（36.4%） and the allele Sat_320-A286,the largest positive effect for PW（31.4%）.

关 键 词：关联分析 遗传多样性 连锁不平衡 抗虫性 大豆 

分 类 号：S565.1[农业科学—作物学]