糖皮质激素受体-α表达下调与鼻息肉相关性的研究  

The correlation between down regulation of glucocorticoid receptor-α mRNA and nasal polyps

作　　者：李鹏[1] 张革化[1] 黄雪琨[1] 李源[1] 

机构地区：[1]中山大学附属第三医院耳鼻咽喉头颈外科,广东广州510630

出　　处：《中国耳鼻咽喉头颈外科》2011年第9期491-494,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的检测糖皮质激素受体α(glucocorticoid receptor-α,GR-α)mRNA在鼻息肉组织及健康人群鼻腔下鼻甲黏膜、窦口鼻道复合体(ostiomeatal complex,OMC)区黏膜的定量表达,并探讨其在鼻息肉发生发展中可能存在的的机制。方法采用荧光定量逆转录-聚合酶链反应(FQ-RT-PCR)检测下鼻甲黏膜、OMC区黏膜及鼻息肉组织中GR-α的定量表达。结果下鼻甲黏膜中GR-αmRNA表达[(135.4±5.2)×104 copies/μg]高于OMC区黏膜[(114.7±4.3)×104 copies/μg],有显著差异(P<0.01);下鼻甲、OMC区黏膜GR-αmRNA表达均高于鼻息肉组织[(23.5±12.1)×104 copies/μg]有显著差异(P<0.01);随着鼻息肉的发展,其组织中的GR-α表达量逐渐减少。结论鼻腔黏膜局部区域GR-α表达下调可能是鼻息肉发生发展的始动因素之一。OBJECTIVE To detect the expression of the glucocorticoid receptor-α（GR-α） mRNA in nasal polyps of patients and nasal mucosa of the inferior turbinate,ostiomeatal complex（OMC）of healthy adults.And to investigate the significance of GR-α in the initiation and progression of nasal polyps.METHODS The quantitative expression of GR-α mRNA in healthy nasal mucosa of the inferior turbinate,OMC and polyps were examined by using Fluorescent quantitative PCR（FQ-RT-PCR）.RESULTS The GR-α mRNA level in normal mucosa of inferior turbinate[（135.4±5.2）×104 copies/μg ]was significantly higher than that of OMC[（114.7±4.3）×104 copies/μg]（P0.01）.The GR-α mRNA level in normal mucosa of inferior turbinate and OMC were significantly higher than that of nasal polyps[（23.5±12.1）×104 copies/μg]（P0.01）.With the development of the nasal polyps,the expression of the GR-α decreased.CONCLUSION Down regulation of the expression of GR-α mRNA in nasal mucosa might be an initiating factor of the progression of nasal polyps.

关 键 词：鼻息肉 糖皮质激素类 受体 糖皮质激素 荧光定量逆转录-聚合酶链反应 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]